PMID- 15630239
OWN - NLM
STAT- MEDLINE
DCOM- 20050418
LR  - 20191026
IS  - 0951-6433 (Print)
IS  - 0951-6433 (Linking)
VI  - 21
IP  - 1-4
DP  - 2004
TI  - Effect of simultaneous administration of vitamin C, L-cysteine and vitamin E on 
      the melanogenesis.
PG  - 415-8
AB  - The effect of simultaneous administration of vitamin C (ascorbic acid), L-cystein 
      (Cys) and vitamin E (tocopherol) on the melanogenesis in vivo and in vitro was 
      studied. Forty-eight brownish guinea pigs were divided into 4 groups as follows: 
      VC group, VC+Cys group, VC+Cys+VE group and control group. They were given these 
      vitamins by oral administration every day. UV-B exposure (0.384 J/cm2) on their 
      depleted back skin was done at the day 8, 10, 12, 15 17 and 19. After UV-B 
      irradiation, vitamins were administrated further 3 weeks. The luminosity score 
      was measured using a Color Reader CR-11 (Minolta, Co) and the numbers of 
      DOPA-positive melanocytes of their back skin were counted. B16 melanoma cells 
      were incubated with VC, N-acetyl cystein (NAC) and VE. After 4 days of 
      incubation, cells were harvested. The melanin contents and the tyrosinase 
      activities in cells were measured. The luminosity score in the VC+VE+Cys group 
      was higher than those in the other groups. The numbers of DOPA-positive 
      melanocytes of guinea pigs treated with VC, VE and Cys were significantly 
      decreased compared with those in VC group. In B16 melanoma cells, simultaneous 
      treatment of VC, VE and NAC was the most effective to decrease the melanin 
      contents and to inhibit tyrosinase activity.
FAU - Fujiwara, Yoko
AU  - Fujiwara Y
AD  - Department of Nutrition and Food Science, Ochanomizu University, 2-1-1, Otsuka, 
      Bunkyo-ku, Tokyo 112-8610, Japan. fujiwara@cc.ocha.ac.jp
FAU - Sahashi, Yumiko
AU  - Sahashi Y
FAU - Aritro, Mitsumi
AU  - Aritro M
FAU - Hasegawa, Satoko
AU  - Hasegawa S
FAU - Akimoto, Koji
AU  - Akimoto K
FAU - Ninomiya, Shinji
AU  - Ninomiya S
FAU - Sakaguchi, Yasue
AU  - Sakaguchi Y
FAU - Seyama, Yousuke
AU  - Seyama Y
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - Biofactors
JT  - BioFactors (Oxford, England)
JID - 8807441
RN  - 0 (Melanins)
RN  - 1406-18-4 (Vitamin E)
RN  - EC 1.14.18.1 (Monophenol Monooxygenase)
RN  - K848JZ4886 (Cysteine)
RN  - PQ6CK8PD0R (Ascorbic Acid)
SB  - IM
MH  - Animals
MH  - Ascorbic Acid/*pharmacology
MH  - Cell Line, Tumor
MH  - Cysteine/*pharmacology
MH  - Guinea Pigs
MH  - Melanins/metabolism
MH  - Melanocytes/drug effects/*physiology
MH  - Melanoma, Experimental
MH  - Monophenol Monooxygenase/antagonists & inhibitors
MH  - Ultraviolet Rays
MH  - Vitamin E/*pharmacology
EDAT- 2005/01/05 09:00
MHDA- 2005/04/19 09:00
CRDT- 2005/01/05 09:00
PHST- 2005/01/05 09:00 [pubmed]
PHST- 2005/04/19 09:00 [medline]
PHST- 2005/01/05 09:00 [entrez]
AID - 10.1002/biof.552210182 [doi]
PST - ppublish
SO  - Biofactors. 2004;21(1-4):415-8. doi: 10.1002/biof.552210182.