PMID- 15630697
OWN - NLM
STAT- MEDLINE
DCOM- 20050222
LR  - 20181201
IS  - 0008-543X (Print)
IS  - 0008-543X (Linking)
VI  - 103
IP  - 3
DP  - 2005 Feb 1
TI  - Protein expression and gene amplification of epidermal growth factor receptor in 
      thymomas.
PG  - 630-6
AB  - BACKGROUND: Epidermal growth factor receptor (EGFR) overexpression and 
      amplification are important prognostic factors in many solid tumors and anti-EGFR 
      antibody-based therapy is now available as a promising therapeutic modality. 
      There is little information in the literature regarding the biologic role of EGFR 
      in thymomas that are characterized by variable clinical presentations, histologic 
      heterogeneity, and unpredictable behavior. METHODS: Protein expression and gene 
      amplification of EGFR were investigated in 32 thymomas (9 World Health 
      Organization [WHO] type A, 5 type AB, 7 type B2, 7 type B3, 4 type C) using 
      immunohistochemistry and fluorescence in situ hybridization (FISH). FISH analysis 
      included assessment of the average number of copies of the EGFR gene per cell, 
      the average ratio of the EGFR gene to chromosome 7 copy numbers, and ploidy. 
      RESULTS: The results of FISH analysis showed statistically significant 
      correlation with WHO histologic type, invasion, advanced clinical stage, but not 
      with tumor size and outcome. Thymomas associated with myasthenia gravis more 
      frequently showed hyperploidy when compared with sporadic tumors, but there was 
      no difference in EGFR gene amplification. EGFR protein expression assessed by 
      immunohistochemistry did not correlate with any studied clinicopathologic 
      variables. There was poor correlation between the protein expression and gene 
      amplification, only 7 of 23 specimens (30%). CONCLUSIONS: The potential role of 
      EGFR in the pathogenesis of advanced-stage thymomas indicated that evolving 
      anti-EGFR antibody therapy may be considered as a treatment option.
CI  - (c) 2004 American Cancer Society
FAU - Ionescu, Diana N
AU  - Ionescu DN
AD  - Division of Anatomic Pathology, Department of Pathology, University of Pittsburgh 
      Medical Center, Presbyterian University Hospital, Pittsburgh, Pennsylvania, USA. 
      ionescudn@upmc.edu
FAU - Sasatomi, Elizaburo
AU  - Sasatomi E
FAU - Cieply, Kathleen
AU  - Cieply K
FAU - Nola, Marin
AU  - Nola M
FAU - Dacic, Sanja
AU  - Dacic S
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Cancer
JT  - Cancer
JID - 0374236
RN  - 0 (Biomarkers, Tumor)
RN  - EC 2.7.10.1 (ErbB Receptors)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Biomarkers, Tumor/*analysis/genetics/immunology
MH  - Chromosomes, Human, Pair 7
MH  - ErbB Receptors/*analysis/genetics/immunology
MH  - Female
MH  - *Gene Amplification
MH  - *Gene Expression Regulation, Neoplastic
MH  - Humans
MH  - Immunohistochemistry
MH  - Immunotherapy/methods
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Middle Aged
MH  - Myasthenia Gravis/complications
MH  - Neoplasm Invasiveness
MH  - Neoplasm Staging
MH  - Ploidies
MH  - Predictive Value of Tests
MH  - Thymoma/*chemistry/complications/immunology/*pathology/therapy
MH  - Tumor Cells, Cultured
EDAT- 2005/01/05 09:00
MHDA- 2005/02/23 09:00
CRDT- 2005/01/05 09:00
PHST- 2005/01/05 09:00 [pubmed]
PHST- 2005/02/23 09:00 [medline]
PHST- 2005/01/05 09:00 [entrez]
AID - 10.1002/cncr.20811 [doi]
PST - ppublish
SO  - Cancer. 2005 Feb 1;103(3):630-6. doi: 10.1002/cncr.20811.