PMID- 15630731
OWN - NLM
STAT- MEDLINE
DCOM- 20050414
LR  - 20211203
IS  - 0315-162X (Print)
IS  - 0315-162X (Linking)
VI  - 32
IP  - 1
DP  - 2005 Jan
TI  - Outer membrane protein of salmonella is the major antigenic target in patients 
      with salmonella induced reactive arthritis.
PG  - 86-92
AB  - OBJECTIVE: We previously reported that Salmonella typhimurium was the triggering 
      agent in one-third of our patients with sporadic enteric reactive arthritis (ReA) 
      and undifferentiated spondyloarthropathy (uSpA). The antigens recognized by the 
      synovial T cells in Salmonella induced ReA are not known. We investigated the 
      immunodominant antigens in Salmonella ReA. METHODS: Synovial fluid mononuclear 
      cells (SFMC) from 53 patients with ReA/uSpA were cultured with crude lysate of S. 
      typhimurium. In 20 patients, the triggering agent was found to be Salmonella 
      (stimulation index, SI, > 2.5). For cell fractionation of S. typhimurium, the 
      sonicated crude lysate was separated by ultracentrifugation into a cytoplasmic 
      supernatant (CYT) and membrane pellet (OMP). The CYT was further separated on 
      SDS-PAGE and blotted onto nitrocellulose membrane for proliferation assays. SFMC 
      from 20 patients with Salmonella ReA/uSpA were stimulated with OMP, CYT, and 
      cytosolic fractions of S. typhimurium, and proliferation was measured by 
      thymidine incorporation. Quantitation of antigen-specific cells in SF was by 
      intracellular interferon-g staining in 7 patients and paired peripheral blood 
      (PB) in 5 patients after stimulation with crude Salmonella lysate, CYT, and OMP. 
      RESULTS: Out of 20 patients with Salmonella ReA/uSpA, the SFMC showed a 
      significant proliferation to OMP in 19 patients and CYT in 17 patients. The 
      median SI of OMP (8.2, range 2.8-52.5) was significantly higher (p < 0.0005) than 
      for the CYT (4.9, 2.7-18.8). Fifty percent of the patients showed proliferative 
      response to cytosolic fractions of < 60 kDa. The mean antigen-specific T cell 
      frequency was also higher with OMP (0.68% +/- 0.59%) than CYT (0.53% +/- 0.62%) 
      but this was not statistically significant. Compared to PB, the OMP-specific 
      cells were 7.5 times more numerous in the SF (p < 0.05). CONCLUSION: The cellular 
      immune response in Salmonella ReA/uSpA is directed predominantly against the OMP 
      and low molecular weight proteins in the cytosolic fraction.
FAU - Saxena, Shweta
AU  - Saxena S
AD  - Department of Immunology, Sanjay Gandhi Postgraduate Institute of Medical 
      Sciences, Lucknow, India.
FAU - Aggarwal, Amita
AU  - Aggarwal A
FAU - Misra, Ramnath
AU  - Misra R
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Canada
TA  - J Rheumatol
JT  - The Journal of rheumatology
JID - 7501984
RN  - 0 (Antigens, Bacterial)
RN  - 0 (Bacterial Proteins)
RN  - 0 (Membrane Proteins)
RN  - 0 (PHB2 protein, human)
RN  - 0 (Prohibitins)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Antigens, Bacterial/analysis/*immunology
MH  - Arthritis, Reactive/*immunology
MH  - Bacterial Proteins/analysis/*immunology
MH  - Cell Proliferation
MH  - Cells, Cultured
MH  - Electrophoresis, Polyacrylamide Gel
MH  - Female
MH  - Humans
MH  - Lymphocyte Activation/immunology
MH  - Male
MH  - Membrane Proteins/analysis/*immunology
MH  - Prohibitins
MH  - Salmonella Infections/*immunology
MH  - Salmonella typhimurium/*immunology
MH  - Synovial Fluid/cytology/immunology
MH  - T-Lymphocytes/immunology
EDAT- 2005/01/05 09:00
MHDA- 2005/04/15 09:00
CRDT- 2005/01/05 09:00
PHST- 2005/01/05 09:00 [pubmed]
PHST- 2005/04/15 09:00 [medline]
PHST- 2005/01/05 09:00 [entrez]
AID - 0315162X-32-86 [pii]
PST - ppublish
SO  - J Rheumatol. 2005 Jan;32(1):86-92.