PMID- 15632010
OWN - NLM
STAT- MEDLINE
DCOM- 20050302
LR  - 20240412
IS  - 0002-9440 (Print)
IS  - 1525-2191 (Electronic)
IS  - 0002-9440 (Linking)
VI  - 166
IP  - 1
DP  - 2005 Jan
TI  - Human herpesvirus 6 activates lytic cycle replication of Kaposi's 
      sarcoma-associated herpesvirus.
PG  - 173-83
AB  - Kaposi's sarcoma-associated herpesvirus (KSHV) or human herpesvirus 8 (HHV-8) is 
      a gamma-herpesvirus consistently identified in Kaposi's sarcoma (KS), primary 
      effusion lymphoma, and multicentric Castleman's disease. KSHV infection appears 
      to be necessary, but not be sufficient for development of KS without other 
      co-factors. However, factors that facilitate KSHV to cause KS have not been well 
      defined. Because patients with KS are often immunosuppressed and susceptible to 
      many infectious agents including human herpesvirus 6 (HHV-6), we investigated the 
      potential of HHV-6 to influence the replication of KSHV. By co-culturing 
      HHV-6-infected T cells with KSHV-latent BCBL-1 cell line, infecting BCBL-1 cells 
      with HHV-6 virions, and generating heterokaryons between HHV-6-infected T cells 
      and BCBL-1 cells, we showed that HHV-6 played a critical role in induction of 
      KSHV replication, as determined by production of lytic phase mRNA transcripts and 
      viral proteins. We confirmed and extended the results by using a luciferase 
      reporter assay in which KSHV ORF50 promoter, the first promoter activated during 
      KSHV replication, drove the luciferase expression. Besides HHV-6, we also found 
      that cytokines such as interferon-gamma partially contributed to induction of 
      KSHV replication in the co-culture system. These findings suggest that HHV-6 may 
      participate in KS pathogenesis by promoting KSHV replication and increasing KSHV 
      viral load.
FAU - Lu, Chun
AU  - Lu C
AD  - Department of Microbiology and Immunology, Nanjing Medical University, Nanjing 
      210029, P. R. China. clu@njmu.edu.cn
FAU - Zeng, Yi
AU  - Zeng Y
FAU - Huang, Zan
AU  - Huang Z
FAU - Huang, Li
AU  - Huang L
FAU - Qian, Chao
AU  - Qian C
FAU - Tang, Guixia
AU  - Tang G
FAU - Qin, Di
AU  - Qin D
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Am J Pathol
JT  - The American journal of pathology
JID - 0370502
SB  - IM
EIN - Am J Pathol. 2021 May;191(5):965. PMID: 33894887
MH  - Cell Cycle/*physiology
MH  - Cell Division/*physiology
MH  - Cell Line
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Flow Cytometry
MH  - *Herpesvirus 6, Human/genetics
MH  - *Herpesvirus 8, Human/genetics
MH  - Humans
MH  - Immunoenzyme Techniques
MH  - Polymerase Chain Reaction
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Sarcoma, Kaposi/*pathology
MH  - Virus Replication/*physiology
PMC - PMC1602294
EDAT- 2005/01/06 09:00
MHDA- 2005/03/03 09:00
PMCR- 2005/07/01
CRDT- 2005/01/06 09:00
PHST- 2005/01/06 09:00 [pubmed]
PHST- 2005/03/03 09:00 [medline]
PHST- 2005/01/06 09:00 [entrez]
PHST- 2005/07/01 00:00 [pmc-release]
AID - S0002-9440(10)62242-0 [pii]
AID - 10.1016/S0002-9440(10)62242-0 [doi]
PST - ppublish
SO  - Am J Pathol. 2005 Jan;166(1):173-83. doi: 10.1016/S0002-9440(10)62242-0.