PMID- 15632448
OWN - NLM
STAT- MEDLINE
DCOM- 20050322
LR  - 20200826
IS  - 1350-0872 (Print)
IS  - 1350-0872 (Linking)
VI  - 151
IP  - Pt 1
DP  - 2005 Jan
TI  - Molecular evolution of Vibrio pathogenicity island-2 (VPI-2): mosaic structure 
      among Vibrio cholerae and Vibrio mimicus natural isolates.
PG  - 311-322
LID - 10.1099/mic.0.27621-0 [doi]
AB  - Vibrio cholerae is a Gram-negative rod that inhabits the aquatic environment and 
      is the aetiological agent of cholera, a disease that is endemic in much of 
      Southern Asia. The 57.3 kb Vibrio pathogenicity island-2 (VPI-2) is confined 
      predominantly to toxigenic V. cholerae O1 and O139 serogroup isolates and encodes 
      52 ORFs (VC1758 to VC1809), which include homologues of an integrase (VC1758), a 
      restriction modification system, a sialic acid metabolism gene cluster 
      (VC1773-VC1783), a neuraminidase (VC1784) and a gene cluster that shows homology 
      to Mu phage. In this study, a 14.1 kb region of VPI-2 comprising ORFs VC1773 to 
      VC1787 was identified by PCR and Southern blot analyses in all 17 Vibrio mimicus 
      isolates examined. The VPI-2 region in V. mimicus was inserted adjacent to a 
      serine tRNA similar to VPI-2 in V. cholerae. In 11 of the 17 V. mimicus isolates 
      examined, an additional 5.3 kb region encoding VC1758 and VC1804 to VC1809 was 
      present adjacent to VC1787. The evolutionary history of VPI-2 was reconstructed 
      by comparative analysis of the nanH (VC1784) gene tree with the species gene 
      tree, deduced from the housekeeping gene malate dehydrogenase (mdh), among V. 
      cholerae and V. mimicus isolates. Both gene trees showed an overall congruence; 
      on both gene trees V. cholerae O1 and O139 serogroup isolates clustered together, 
      whereas non-O1/non-O139 serogroup isolates formed separate divergent branches 
      with similar clustering of strains within the branches. One exception was noted: 
      on the mdh gene tree, V. mimicus sequences formed a distinct divergent lineage 
      from V. cholerae sequences; however, on the nanH gene tree, V. mimicus clustered 
      with V. cholerae non-O1/non-O139 isolates, suggesting horizontal transfer of this 
      region between these species.
FAU - Jermyn, William S
AU  - Jermyn WS
AD  - Department of Microbiology, UCC, National University of Ireland-Cork, Cork, 
      Ireland.
FAU - Boyd, E Fidelma
AU  - Boyd EF
AD  - Department of Microbiology, UCC, National University of Ireland-Cork, Cork, 
      Ireland.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Microbiology (Reading)
JT  - Microbiology (Reading, England)
JID - 9430468
RN  - 0 (Bacterial Proteins)
SB  - IM
MH  - Bacterial Proteins/chemistry/genetics/metabolism
MH  - Base Sequence
MH  - Chromosome Mapping
MH  - *Evolution, Molecular
MH  - Genetic Variation
MH  - Genomic Islands/*genetics
MH  - Humans
MH  - Phylogeny
MH  - Vibrio cholerae/genetics/*pathogenicity
MH  - Vibrio mimicus/genetics/*pathogenicity
EDAT- 2005/01/06 09:00
MHDA- 2005/03/23 09:00
CRDT- 2005/01/06 09:00
PHST- 2005/01/06 09:00 [pubmed]
PHST- 2005/03/23 09:00 [medline]
PHST- 2005/01/06 09:00 [entrez]
AID - 10.1099/mic.0.27621-0 [doi]
PST - ppublish
SO  - Microbiology (Reading). 2005 Jan;151(Pt 1):311-322. doi: 10.1099/mic.0.27621-0.