PMID- 15633221
OWN - NLM
STAT- MEDLINE
DCOM- 20050222
LR  - 20190430
IS  - 1007-9327 (Print)
IS  - 2219-2840 (Electronic)
IS  - 1007-9327 (Linking)
VI  - 11
IP  - 2
DP  - 2005 Jan 14
TI  - Expression of fragile histidine triad in primary hepatocellular carcinoma and its 
      relation with cell proliferation and apoptosis.
PG  - 228-31
AB  - AIM: To evaluate the expression of fragile histidine triad (FHIT) gene protein, 
      product of a candidate tumor suppressor, and to investigate the relationship 
      between FHIT, cell apoptosis and proliferation, and pathological features of 
      primary hepatocellular carcinoma (HCC). METHODS: Forty-seven HCC and ten normal 
      liver specimens were collected during surgical operation between 2001 and 2003. 
      FHIT and proliferating cell nuclear antigen (PCNA) expression were detected by 
      immunohistochemistry, and apoptotic level was evaluated by terminal 
      deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) assay on the 
      tissue sections. RESULTS: All normal liver tissues showed a strong expression of 
      FHIT, whereas 28 of 47 (59.6%) carcinomas showed a significant loss or absence of 
      FHIT expression (P = 0.001). The proportion of reduced FHIT expression in those 
      carcinomas at stages III-IV (70.6%) and in those with extrahepatic metastasis 
      (86.7%) showed an increasing trend compared with those at stages I-II (30.8%, P = 
      0.013) and those without metastasis (46.9%, P = 0.010) respectively. Apoptotic 
      incidence in advanced TNM stage carcinoma and those with positive FHIT expression 
      was higher than that in early stage carcinoma (P = 0.030) and in those with 
      negative FHIT expression (P = 0.044) respectively. The proliferating potential of 
      hepatocellular carcinoma was associated with FHIT expression (P = 0.016) and the 
      aggressive feature (P = 0.019). Kaplan-Meier analysis demonstrated that the 
      survival time of these 47 patients correlated with TNM stage, FHIT expression and 
      metastasis. CONCLUSION: There is marked loss or absence of FHIT expression, as 
      well as abnormal apoptosis-proliferation balance in HCC. FHIT may play an 
      important role in carcinogenesis and development of HCC.
FAU - Nan, Ke-Jun
AU  - Nan KJ
AD  - Department of Oncology, First Hospital of Xi'an Jiaotong University, Xi'an 
      710061, Shaanxi Province, China.
FAU - Ruan, Zhi-Ping
AU  - Ruan ZP
FAU - Jing, Zhao
AU  - Jing Z
FAU - Qin, Hai-Xia
AU  - Qin HX
FAU - Wang, Hong-Yan
AU  - Wang HY
FAU - Guo, Hui
AU  - Guo H
FAU - Xu, Rui
AU  - Xu R
LA  - eng
PT  - Journal Article
PL  - United States
TA  - World J Gastroenterol
JT  - World journal of gastroenterology
JID - 100883448
RN  - 0 (Neoplasm Proteins)
RN  - 0 (fragile histidine triad protein)
RN  - 4QD397987E (Histidine)
RN  - EC 3.6.- (Acid Anhydride Hydrolases)
SB  - IM
MH  - Acid Anhydride Hydrolases/*genetics
MH  - Adult
MH  - Apoptosis/*genetics
MH  - Carcinoma, Hepatocellular/*genetics/*pathology
MH  - Cell Division/*genetics
MH  - Female
MH  - *Genes, Tumor Suppressor
MH  - Histidine/*genetics
MH  - Humans
MH  - Liver/physiology
MH  - Liver Neoplasms/*genetics/*pathology
MH  - Male
MH  - Middle Aged
MH  - Neoplasm Metastasis
MH  - Neoplasm Proteins/*genetics
MH  - Neoplasm Staging
MH  - Reference Values
PMC - PMC4205407
EDAT- 2005/01/06 09:00
MHDA- 2005/02/23 09:00
PMCR- 2005/01/14
CRDT- 2005/01/06 09:00
PHST- 2005/01/06 09:00 [pubmed]
PHST- 2005/02/23 09:00 [medline]
PHST- 2005/01/06 09:00 [entrez]
PHST- 2005/01/14 00:00 [pmc-release]
AID - 10.3748/wjg.v11.i2.228 [doi]
PST - ppublish
SO  - World J Gastroenterol. 2005 Jan 14;11(2):228-31. doi: 10.3748/wjg.v11.i2.228.