PMID- 15633635
OWN - NLM
STAT- MEDLINE
DCOM- 20050126
LR  - 20220317
IS  - 0379-4172 (Print)
IS  - 0379-4172 (Linking)
VI  - 31
IP  - 12
DP  - 2004 Dec
TI  - [Expression and promoter methylation of Apaf-1 gene in laryngeal squamous cell 
      carcinoma].
PG  - 1327-31
AB  - Loss of tumor suppressor genes and apoptosis-associated genes was common event in 
      laryngeal squamous cell carcinoma (LSCC). Apaf-1 (apoptotic protease activating 
      factor-1) is a key factor in cytochrome C-dependent apoptotic pathway. To 
      investigate the effect of Apaf-1 in progression of LSCC, we analyzed Apaf-1 from 
      DNA and RNA levels. Semi-quantitative RT-PCR analysis of Apaf-1 mRNA showed that 
      over 40 percent of LSCCs (11/27) were low expression compared to the 
      para-neoplastic laryngeal tissues (PNTs). The results of CGH indicated loss in 2 
      of 18 cases but no amplification on chromosome 12q22-23. The LOH frequencies of 
      D12S327, D12S1657, D12S393, D12S1706, D12S346 on 12q22-23 were 18.2%, 13.6%, 
      18.2%, 22.2% and 16.6%, respectively in 72 matched samples of LSCCs and PNTs. 
      Methylation-specific PCR displayed that all of 11 LSCCs, whose expression of 
      Apaf-1 mRNA down-regulated, were methylated in promoter regions. In contrast, 
      only 1 of 16 LSCCs with no changes in Apaf-1 mRNA levels was methylated (chi2 
      test, P=0.0001). The results implied that abnormal expression of Apaf-1 
      participates in the genesis of LSCCs and decreased expression of Apaf-1 mRNA were 
      not mainly due to the deletion of Apaf-1 gene. The Apaf-1 DNA methylation in 
      promoter region might contribute to the decreased transcription of Apaf-1 in 
      LSCCs.
FAU - Huang, Dai-Fa
AU  - Huang DF
AD  - Department of Medical Genetics, China Medical University, Shenyang 110001, China.
FAU - Fu, Wei-Neng
AU  - Fu WN
FAU - Shang, Chao
AU  - Shang C
FAU - Xu, Zhen-Ming
AU  - Xu ZM
FAU - Li, Zeng-Gang
AU  - Li ZG
FAU - Sun, Kai-Lai
AU  - Sun KL
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Yi Chuan Xue Bao
JT  - Yi chuan xue bao = Acta genetica Sinica
JID - 7900784
RN  - 0 (APAF1 protein, human)
RN  - 0 (Apoptotic Protease-Activating Factor 1)
RN  - 0 (Proteins)
SB  - IM
MH  - Apoptotic Protease-Activating Factor 1
MH  - Carcinoma, Squamous Cell/*genetics
MH  - Chromosomes, Human, Pair 12
MH  - CpG Islands
MH  - *DNA Methylation
MH  - Humans
MH  - Laryngeal Neoplasms/*genetics
MH  - *Promoter Regions, Genetic
MH  - Proteins/*genetics
EDAT- 2005/01/07 09:00
MHDA- 2005/01/27 09:00
CRDT- 2005/01/07 09:00
PHST- 2005/01/07 09:00 [pubmed]
PHST- 2005/01/27 09:00 [medline]
PHST- 2005/01/07 09:00 [entrez]
PST - ppublish
SO  - Yi Chuan Xue Bao. 2004 Dec;31(12):1327-31.