PMID- 15634404
OWN - NLM
STAT- MEDLINE
DCOM- 20061013
LR  - 20131121
IS  - 0529-5815 (Print)
IS  - 0529-5815 (Linking)
VI  - 42
IP  - 22
DP  - 2004 Nov 22
TI  - [Relationship between estradiol and the mitogenic activated protein kinase signal 
      transduction pathway].
PG  - 1363-6
AB  - OBJECTIVE: To discuss the relationship between estradiol and the mitogenic 
      activated protein kinase signal transduction pathway and the expression of the 
      MAPK in the MCF-7 breast cancer cell-line. METHODS: Epithelial growth factor 
      (EGF) and different concentration of estradiol to induce the expression of 
      phosphospecific ERK1/2 (pERK1/2) in MCF-7 cell line was used and the expression 
      of pERK1/2 with western-blotting was detected. Then antiestrogen ICI 182780 and 
      MAPK inhibitor PD98059 to inhibit the expression of pERK1/2 was used. The cell 
      cycle of MCF-7 was detected by FACS. RESULTS: EGF could significantly induce the 
      expression of pERK1/2. Estradiol could also induce the expression of pERK1/2, but 
      the intensity was less than the induction of EGF. The percentage of cells in the 
      G(2)/M cell cycle after estradiol induction increased (18.38%) compared to the 
      control group (10.52%) (P < 0.05). CONCLUSIONS: MAPK is an important regulatory 
      signal in breast cancer. Its measurement in breast cancer tissues provides 
      information about the degree of activation of various growth factor pathways. 
      This molecule may also provide a molecular target for compounds designed to block 
      cell proliferation.
FAU - Chen, Jian-Ying
AU  - Chen JY
AD  - Department of General Surgery, Union Hospital, Tongji Medical College, Huazhong 
      Science and Technology University, Wuhan 430022, China.
FAU - Zhang, Bo
AU  - Zhang B
FAU - Wang, Guo-Bin
AU  - Wang GB
FAU - Chen, Dao-da
AU  - Chen DD
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Zhonghua Wai Ke Za Zhi
JT  - Zhonghua wai ke za zhi [Chinese journal of surgery]
JID - 0153611
RN  - 0 (Receptors, Estrogen)
RN  - 4TI98Z838E (Estradiol)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinases)
SB  - IM
MH  - Breast Neoplasms/enzymology/*pathology/physiopathology
MH  - Cell Proliferation/*drug effects
MH  - Estradiol/*pharmacology
MH  - Female
MH  - Humans
MH  - MAP Kinase Signaling System/drug effects/*physiology
MH  - Mitogen-Activated Protein Kinases/drug effects/*metabolism
MH  - Receptors, Estrogen/metabolism
MH  - Signal Transduction
MH  - Tumor Cells, Cultured
EDAT- 2005/01/07 09:00
MHDA- 2006/10/14 09:00
CRDT- 2005/01/07 09:00
PHST- 2005/01/07 09:00 [pubmed]
PHST- 2006/10/14 09:00 [medline]
PHST- 2005/01/07 09:00 [entrez]
PST - ppublish
SO  - Zhonghua Wai Ke Za Zhi. 2004 Nov 22;42(22):1363-6.