PMID- 15634454
OWN - NLM
STAT- MEDLINE
DCOM- 20070320
LR  - 20160818
IS  - 0529-5807 (Print)
IS  - 0529-5807 (Linking)
VI  - 33
IP  - 6
DP  - 2004 Dec
TI  - [Induction of monocyte chemoattractant protein-1 expression in human mesangial 
      cells by angiotensin II: role of c-Jun N-terminal kinase-c-Jun/activator 
      protein-1 signal pathway].
PG  - 550-4
AB  - OBJECTIVE: To investigate the role of c-Jun N-terminal kinase 
      (JNK)-c-Jun/activator protein-1 (AP-1) signal pathway in expression of monocyte 
      chemoattractant protein-1 (MCP-1) in experimental rat glomerulonephritis. 
      METHODS: Nephrotoxic sera nephritis (NTN) was induced by injection of anti-GBM 
      antibody into the tail veins of rats. Electrophoretic mobility shift assay (EMSA) 
      and non-radioactive kinase assay were used to detect the activity of AP-1 and JNK 
      in kidneys and angiotensin II-stimulated human mesangial cells. Ribonuclear 
      protection assay was used to detect MCP-1 expression in cultured human mesangial 
      cells. RESULTS: Significant up-regulation of JNK and AP-1 was observed in NTN 
      rats (3.82 +/- 0.58) folds and (5.36 +/- 0.61) folds, as compared with the 
      controls. Supershift assay demonstrated that c-Jun and c-Fos were the predominant 
      subunits involved. Activation of JNK and AP-1 significantly correlated with MCP-1 
      expression in NTN rats. Angiotensin II enhanced the expression of MCP-1 and 
      activation of JNK and AP-1 in cultured human mesangial cells in a dose-dependent 
      manner, with maximal stimulation seen at 100 nmol/L (20.99 +/- 4.71) folds, (6.91 
      +/- 1.65) folds and (7.82 +/- 1.32) folds respectively. Significant 
      down-regulation of AP-1 activation and MCP-1 expression were observed in 
      angiotensin II-induced human mesangial cells pretreated with JNK specific 
      inhibitor SP600125. CONCLUSIONS: Angiotensin II and MCP-1 may play an important 
      role in glomerulosclerosis via the JNK-c-Jun/AP-1 signal pathway.
FAU - Zhang, Ai-Hua
AU  - Zhang AH
AD  - Department of Nephrology, Nanjing Children's Hospital, Affiliated to Nanjing 
      Medical University, Nanjing 210008, China.
FAU - Huang, Song-Ming
AU  - Huang SM
FAU - Ding, Gui-Xia
AU  - Ding GX
FAU - Wu, Yuan-Jun
AU  - Wu YJ
FAU - Zhang, Wei-Zhen
AU  - Zhang WZ
FAU - Wu, Hong-Mei
AU  - Wu HM
FAU - Fei, Li
AU  - Fei L
FAU - Guo, Mei
AU  - Guo M
FAU - Chen, Rong-Hua
AU  - Chen RH
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Zhonghua Bing Li Xue Za Zhi
JT  - Zhonghua bing li xue za zhi = Chinese journal of pathology
JID - 0005331
RN  - 0 (Chemokine CCL2)
RN  - 0 (Proto-Oncogene Proteins c-fos)
RN  - 0 (Proto-Oncogene Proteins c-jun)
RN  - 0 (Transcription Factor AP-1)
RN  - 11128-99-7 (Angiotensin II)
RN  - EC 2.7.11.24 (JNK Mitogen-Activated Protein Kinases)
SB  - IM
MH  - Angiotensin II/*pharmacology
MH  - Animals
MH  - Cells, Cultured
MH  - Chemokine CCL2/*metabolism
MH  - Glomerular Mesangium/cytology/*metabolism
MH  - Glomerulonephritis/metabolism
MH  - Humans
MH  - JNK Mitogen-Activated Protein Kinases/*metabolism
MH  - Male
MH  - Proto-Oncogene Proteins c-fos/metabolism
MH  - Proto-Oncogene Proteins c-jun/metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Signal Transduction/drug effects
MH  - Transcription Factor AP-1/*metabolism
EDAT- 2005/01/07 09:00
MHDA- 2007/03/21 09:00
CRDT- 2005/01/07 09:00
PHST- 2005/01/07 09:00 [pubmed]
PHST- 2007/03/21 09:00 [medline]
PHST- 2005/01/07 09:00 [entrez]
PST - ppublish
SO  - Zhonghua Bing Li Xue Za Zhi. 2004 Dec;33(6):550-4.