PMID- 15635594
OWN - NLM
STAT- MEDLINE
DCOM- 20050418
LR  - 20161124
IS  - 0360-4012 (Print)
IS  - 0360-4012 (Linking)
VI  - 79
IP  - 4
DP  - 2005 Feb 15
TI  - Transport of neurofilaments in growing axons requires microtubules but not actin 
      filaments.
PG  - 442-50
AB  - Neurofilament (NF) polymers are conveyed from cell body to axon tip by slow 
      axonal transport, and disruption of this process is implicated in several 
      neuronal pathologies. This movement occurs in both anterograde and retrograde 
      directions and is characterized by relatively rapid but brief movements of 
      neurofilaments, interrupted by prolonged pauses. The present studies combine 
      pharmacologic treatments that target actin filaments or microtubules with imaging 
      of NF polymer transport in living axons to examine the dependence of 
      neurofilament transport on these cytoskeletal systems. The heavy NF subunit 
      tagged with green fluorescent protein was expressed in cultured sympathetic 
      neurons to visualize NF transport. Depletion of axonal actin filaments by 
      treatment with 5 microM latrunculin for 6 hr had no detectable effect on 
      directionality or transport rate of NFs, but frequency of movement events was 
      reduced from 1/3.1 min of imaging time to 1/4.9 min. Depolymerization of axonal 
      microtubules using either 5 microM vinblastine for 3 hr or 5 microg/ml nocodazole 
      for 4-6 hr profoundly suppressed neurofilament transport. In 92% of treated 
      neurons, NF transport was undetected. These observations indicate that actin 
      filaments are not required for neurofilament transport, although they may have 
      subtle effects on neurofilament movements. In contrast, axonal transport of NFs 
      requires microtubules, suggesting that anterograde and retrograde NF transport is 
      powered by microtubule-based motors.
CI  - Copyright (c) 2005 Wiley-Liss, Inc.
FAU - Francis, Franto
AU  - Francis F
AD  - Department of Anatomy and Cell Biology, Temple University School of Medicine, 
      Philadelphia, Pennsylvania 19140, USA.
FAU - Roy, Subhojit
AU  - Roy S
FAU - Brady, Scott T
AU  - Brady ST
FAU - Black, Mark M
AU  - Black MM
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Neurosci Res
JT  - Journal of neuroscience research
JID - 7600111
RN  - 0 (Bridged Bicyclo Compounds, Heterocyclic)
RN  - 0 (Neurofilament Proteins)
RN  - 0 (Thiazoles)
RN  - 0 (Thiazolidines)
RN  - 147336-22-9 (Green Fluorescent Proteins)
RN  - 5V9KLZ54CY (Vinblastine)
RN  - SH1WY3R615 (Nocodazole)
RN  - SRQ9WWM084 (latrunculin A)
SB  - IM
MH  - Actin Cytoskeleton/*physiology
MH  - Animals
MH  - Animals, Newborn
MH  - Axons/drug effects/*physiology
MH  - Bridged Bicyclo Compounds, Heterocyclic/pharmacology
MH  - Diagnostic Imaging/methods
MH  - Fluorescent Antibody Technique/methods
MH  - Green Fluorescent Proteins/metabolism
MH  - Microtubules/*physiology
MH  - Neurofilament Proteins/*metabolism
MH  - Neurons/drug effects/*physiology
MH  - Nocodazole/pharmacology
MH  - Protein Transport/physiology
MH  - Rats
MH  - Superior Cervical Ganglion/cytology
MH  - Thiazoles/pharmacology
MH  - Thiazolidines
MH  - Time Factors
MH  - Transfection/methods
MH  - Vinblastine/pharmacology
EDAT- 2005/01/07 09:00
MHDA- 2005/04/19 09:00
CRDT- 2005/01/07 09:00
PHST- 2005/01/07 09:00 [pubmed]
PHST- 2005/04/19 09:00 [medline]
PHST- 2005/01/07 09:00 [entrez]
AID - 10.1002/jnr.20399 [doi]
PST - ppublish
SO  - J Neurosci Res. 2005 Feb 15;79(4):442-50. doi: 10.1002/jnr.20399.