PMID- 15636184
OWN - NLM
STAT- MEDLINE
DCOM- 20050216
LR  - 20131121
IS  - 0944-7113 (Print)
IS  - 0944-7113 (Linking)
VI  - 11
IP  - 7-8
DP  - 2004 Nov
TI  - Triterpenoid cynarasaponins from Cynara cardunculus L. reduce chemically induced 
      mutagenesis in vitro.
PG  - 673-8
AB  - Saponins, steroid or triterpene glycosides, are known to have a broad spectrum of 
      biological and pharmacological activities. Three different triterpenoid saponins, 
      marked here as 1s, 2s and 3s, from involucral bracts of Cynara cardunculus L. 
      were isolated and their antimutagenic effect was assessed. Using 
      spectrophotometric method it was shown that all three substances, 1s, 2s and 3s, 
      possess very good absorptive capability. The antimutagenic effect of these 
      substances was estimated against acridine orange (AO)- and ofloxacin-induced 
      damage of chloroplast DNA in Euglena gracilis assay. These cynarasaponins were 
      experimentally confirmed to exhibit different, statistically significant activity 
      in reducing damage of chloroplast DNA of the flagellate E. gracilis induced by AO 
      and ofloxacin (p(t) < 0.05-0.01). Our findings suggest that the antimutagenic 
      effect of 1s, 2s and 3s against AO-induced chloroplast DNA impairment could be a 
      result of their absorptive capacity. As far as ofloxacin is concerned, a possible 
      mechanism of the reduction of the chloroplast DNA lesion was not elucidated so 
      far. To our knowledge, these data demonstrate for the first time the antimutagnic 
      activity of saponins isolated from involucral bracts of C. cardunculus exerted 
      through different mode of action.
FAU - Krizkova, L
AU  - Krizkova L
AD  - Institute of Cell Biology, Faculty of Natural Sciences, Comenius University, 
      Odborarske, namestie 5, 811 07 Bratislava, Slovak Republic. 
      krizkova@m2.fedu.uniba.sk
FAU - Mucaji, P
AU  - Mucaji P
FAU - Nagy, M
AU  - Nagy M
FAU - Krajcovic, J
AU  - Krajcovic J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Germany
TA  - Phytomedicine
JT  - Phytomedicine : international journal of phytotherapy and phytopharmacology
JID - 9438794
RN  - 0 (Antimutagenic Agents)
RN  - 0 (Saponins)
RN  - 0 (Triterpenes)
RN  - A4P49JAZ9H (Ofloxacin)
RN  - F30N4O6XVV (Acridine Orange)
SB  - IM
MH  - Acridine Orange/toxicity
MH  - Animals
MH  - Antimutagenic Agents/isolation & purification/*pharmacology
MH  - Cynara/*chemistry
MH  - Euglena gracilis/drug effects/genetics
MH  - Molecular Structure
MH  - Ofloxacin/antagonists & inhibitors/toxicity
MH  - Saponins/isolation & purification/*pharmacology
MH  - Triterpenes/isolation & purification/*pharmacology
EDAT- 2005/01/08 09:00
MHDA- 2005/02/17 09:00
CRDT- 2005/01/08 09:00
PHST- 2005/01/08 09:00 [pubmed]
PHST- 2005/02/17 09:00 [medline]
PHST- 2005/01/08 09:00 [entrez]
AID - S0944-7113(04)00092-3 [pii]
AID - 10.1016/j.phymed.2003.09.001 [doi]
PST - ppublish
SO  - Phytomedicine. 2004 Nov;11(7-8):673-8. doi: 10.1016/j.phymed.2003.09.001.