PMID- 15638716
OWN - NLM
STAT- MEDLINE
DCOM- 20050524
LR  - 20191109
IS  - 1566-5232 (Print)
IS  - 1566-5232 (Linking)
VI  - 5
IP  - 1
DP  - 2005 Feb
TI  - T cell suicide gene therapy to aid haematopoietic stem cell transplantation.
PG  - 121-32
AB  - Graft versus host disease (GVHD) is a T cell mediated phenomenon that arises 
      following allogeneic haematopoietic stem cell transplantation, and may be 
      particularly severe in the context of human leukocyte antigen (HLA) mismatched 
      procedures. Although GVHD can be largely abrogated through T cell depletion, such 
      measures result in loss of graft potency and reduced anti-viral and 
      anti-leukaemic effects. The genetic modification of T cells to carry a suicide 
      gene mechanism has been advocated as means of allowing T cells to be harnessed 
      for their beneficial effects, and safely eliminated in the event of significant 
      GVHD. The feasibility of the strategy has been demonstrated in clinical studies 
      using T cells modified by retroviral transduction to encode the herpes simplex 
      thymidine kinase (HSVTK) gene to treat patients with haematological malignancies. 
      However, a number of limitations associated with current protocols have become 
      apparent. Most notably, the process of retroviral transduction, which requires 
      pre-activation of T cells, appears to impair subsequent functional potential. 
      Efforts are now directed towards circumventing the pre-activation requirements of 
      retroviral vectors by using alternative lentiviral systems, in association with 
      improved suicide gene/prodrug combinations.
FAU - Qasim, W
AU  - Qasim W
AD  - Molecular Immunology Unit, Institute of Child Health, 30, Guilford Street, London 
      WC1N 1EH, UK. W.Qasim@ich.ucl.ac.uk
FAU - Gaspar, H B
AU  - Gaspar HB
FAU - Thrasher, A J
AU  - Thrasher AJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Review
PL  - United Arab Emirates
TA  - Curr Gene Ther
JT  - Current gene therapy
JID - 101125446
SB  - IM
MH  - Animals
MH  - *Genes, Transgenic, Suicide
MH  - Genetic Therapy/*methods
MH  - *Hematopoietic Stem Cell Transplantation
MH  - Humans
MH  - T-Lymphocytes/*immunology
RF  - 69
EDAT- 2005/01/11 09:00
MHDA- 2005/05/25 09:00
CRDT- 2005/01/11 09:00
PHST- 2005/01/11 09:00 [pubmed]
PHST- 2005/05/25 09:00 [medline]
PHST- 2005/01/11 09:00 [entrez]
AID - 10.2174/1566523052997497 [doi]
PST - ppublish
SO  - Curr Gene Ther. 2005 Feb;5(1):121-32. doi: 10.2174/1566523052997497.