PMID- 15638841
OWN - NLM
STAT- MEDLINE
DCOM- 20050301
LR  - 20061115
IS  - 0903-4641 (Print)
IS  - 0903-4641 (Linking)
VI  - 112
IP  - 11-12
DP  - 2004 Nov-Dec
TI  - Let them fly or light them up: matrix-assisted laser desorption/ionization time 
      of flight (MALDI-TOF) mass spectrometry and fluorescence in situ hybridization 
      (FISH).
PG  - 856-85
AB  - This review focuses on clinical bacteriology and by and large does not cover the 
      detection of fungi, viruses or parasites. It discusses two completely different 
      but complementary approaches that may either supplement or replace classic 
      culture-based bacteriology. The latter view may appear provocative in the light 
      of the actual market penetration of molecular genetic testing in clinical 
      bacteriology. Despite its elegance, high specificity and sensitivity, molecular 
      genetic diagnostics has not yet reached the majority of clinical laboratories. 
      The reasons for this are manifold: Many microbiologists and medical technologists 
      are more familiar with classical microbiological methods than with molecular 
      biology techniques. Culture-based methods still represent the work horse of 
      everyday routine. The number of available FDA-approved molecular genetic tests is 
      limited and external quality control is still under development. Finally, it 
      appears difficult to incorporate genetic testing in the routine laboratory 
      setting due to the limited number of samples received or the lack of appropriate 
      resources. However, financial and time constraints, particularly in hospitals as 
      a consequence of budget cuts and reduced length of stay, lead to a demand for 
      significantly shorter turnaround times that cannot be met by culture-dependent 
      diagnosis. As a consequence, smaller laboratories that do not have the technical 
      and personal equipment required for molecular genetic amplification techniques 
      may adopt alternative methods such as fluorescence in situ hybridization (FISH) 
      that combines easy-to-perform molecular hybridization with microscopy, a 
      technique familiar to every microbiologist. FISH is hence one of the technologies 
      presented here. For large hospital or reference laboratories with a high sample 
      volume requiring massive parallel high-throughput testing we discuss 
      matrix-assisted laser desorption ionization time-of-flight mass spectrometry 
      (MALDI-TOF) of nucleic acids, a technology that has evolved from the post-genome 
      sequencing era, for high-throughput sequence variation analysis (1, 2).
FAU - Schweickert, Birgitta
AU  - Schweickert B
AD  - Institut fur Mikrobiologie und Hygiene, Charite, Universitatsmedizin Berlin, 
      Germany.
FAU - Moter, Annette
AU  - Moter A
FAU - Lefmann, Michael
AU  - Lefmann M
FAU - Gobel, Ulf B
AU  - Gobel UB
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Review
PL  - Denmark
TA  - APMIS
JT  - APMIS : acta pathologica, microbiologica, et immunologica Scandinavica
JID - 8803400
RN  - 0 (RNA, Bacterial)
RN  - 0 (RNA, Ribosomal, 16S)
SB  - IM
MH  - Bacteria/*genetics
MH  - Bacterial Infections/diagnosis/microbiology
MH  - Bacteriological Techniques/*methods
MH  - Gastrointestinal Tract/microbiology
MH  - Genome, Bacterial
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Mouth/microbiology
MH  - RNA, Bacterial/chemistry/genetics
MH  - RNA, Ribosomal, 16S/genetics
MH  - Respiratory System/microbiology
MH  - Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization/*methods
RF  - 308
EDAT- 2005/01/11 09:00
MHDA- 2005/03/02 09:00
CRDT- 2005/01/11 09:00
PHST- 2005/01/11 09:00 [pubmed]
PHST- 2005/03/02 09:00 [medline]
PHST- 2005/01/11 09:00 [entrez]
AID - APMapm11211-1210 [pii]
AID - 10.1111/j.1600-0463.2004.apm11211-1210.x [doi]
PST - ppublish
SO  - APMIS. 2004 Nov-Dec;112(11-12):856-85. doi: 
      10.1111/j.1600-0463.2004.apm11211-1210.x.