PMID- 15640223
OWN - NLM
STAT- MEDLINE
DCOM- 20050315
LR  - 20181113
IS  - 0099-2240 (Print)
IS  - 1098-5336 (Electronic)
IS  - 0099-2240 (Linking)
VI  - 71
IP  - 1
DP  - 2005 Jan
TI  - Diversity and distribution of methanotrophic archaea at cold seeps.
PG  - 467-79
AB  - In this study we investigated by using 16S rRNA-based methods the distribution 
      and biomass of archaea in samples from (i) sediments above outcropping methane 
      hydrate at Hydrate Ridge (Cascadia margin off Oregon) and (ii) massive microbial 
      mats enclosing carbonate reefs (Crimea area, Black Sea). The archaeal diversity 
      was low in both locations; there were only four (Hydrate Ridge) and five (Black 
      Sea) different phylogenetic clusters of sequences, most of which belonged to the 
      methanotrophic archaea (ANME). ANME group 2 (ANME-2) sequences were the most 
      abundant and diverse sequences at Hydrate Ridge, whereas ANME-1 sequences 
      dominated the Black Sea mats. Other seep-specific sequences belonged to the newly 
      defined group ANME-3 (related to Methanococcoides spp.) and to the Crenarchaeota 
      of marine benthic group B. Quantitative analysis of the samples by fluorescence 
      in situ hybridization (FISH) showed that ANME-1 and ANME-2 co-occurred at the 
      cold seep sites investigated. At Hydrate Ridge the surface sediments were 
      dominated by aggregates consisting of ANME-2 and members of the 
      Desulfosarcina-Desulfococcus branch (DSS) (ANME-2/DSS aggregates), which 
      accounted for >90% of the total cell biomass. The numbers of ANME-1 cells 
      increased strongly with depth; these cells accounted 1% of all single cells at 
      the surface and more than 30% of all single cells (5% of the total cells) in 7- 
      to 10-cm sediment horizons that were directly above layers of gas hydrate. In the 
      Black Sea microbial mats ANME-1 accounted for about 50% of all cells. ANME-2/DSS 
      aggregates occurred in microenvironments within the mat but accounted for only 1% 
      of the total cells. FISH probes for the ANME-2a and ANME-2c subclusters were 
      designed based on a comparative 16S rRNA analysis. In Hydrate Ridge sediments 
      ANME-2a/DSS and ANME-2c/DSS aggregates differed significantly in morphology and 
      abundance. The relative abundance values for these subgroups were remarkably 
      different at Beggiatoa sites (80% ANME-2a, 20% ANME-2c) and Calyptogena sites 
      (20% ANME-2a, 80% ANME-2c), indicating that there was preferential selection of 
      the groups in the two habitats. These variations in the distribution, diversity, 
      and morphology of methanotrophic consortia are discussed with respect to the 
      presence of microbial ecotypes, niche formation, and biogeography.
FAU - Knittel, Katrin
AU  - Knittel K
AD  - Max Planck Institute for Marine Microbiology, Department of Molecular Ecology, 
      Celsiusstrasse 1, 28359 Bremen, Germany. kknittel@mpi-bremen.de
FAU - Losekann, Tina
AU  - Losekann T
FAU - Boetius, Antje
AU  - Boetius A
FAU - Kort, Renate
AU  - Kort R
FAU - Amann, Rudolf
AU  - Amann R
LA  - eng
SI  - GENBANK/AJ578027
SI  - GENBANK/AJ578028
SI  - GENBANK/AJ578029
SI  - GENBANK/AJ578030
SI  - GENBANK/AJ578031
SI  - GENBANK/AJ578032
SI  - GENBANK/AJ578033
SI  - GENBANK/AJ578083
SI  - GENBANK/AJ578084
SI  - GENBANK/AJ578085
SI  - GENBANK/AJ578086
SI  - GENBANK/AJ578087
SI  - GENBANK/AJ578088
SI  - GENBANK/AJ578089
SI  - GENBANK/AJ578090
SI  - GENBANK/AJ578091
SI  - GENBANK/AJ578092
SI  - GENBANK/AJ578093
SI  - GENBANK/AJ578094
SI  - GENBANK/AJ578095
SI  - GENBANK/AJ578096
SI  - GENBANK/AJ578097
SI  - GENBANK/AJ578098
SI  - GENBANK/AJ578099
SI  - GENBANK/AJ578100
SI  - GENBANK/AJ578101
SI  - GENBANK/AJ578102
SI  - GENBANK/AJ578103
SI  - GENBANK/AJ578104
SI  - GENBANK/AJ578105
SI  - GENBANK/AJ578106
SI  - GENBANK/AJ578107
SI  - GENBANK/AJ578108
SI  - GENBANK/AJ578109
SI  - GENBANK/AJ578110
SI  - GENBANK/AJ578111
SI  - GENBANK/AJ578112
SI  - GENBANK/AJ578113
SI  - GENBANK/AJ578114
SI  - GENBANK/AJ578115
SI  - GENBANK/AJ578116
SI  - GENBANK/AJ578117
SI  - GENBANK/AJ578118
SI  - GENBANK/AJ578119
SI  - GENBANK/AJ578120
SI  - GENBANK/AJ578121
SI  - GENBANK/AJ578122
SI  - GENBANK/AJ578123
SI  - GENBANK/AJ578124
SI  - GENBANK/AJ578125
SI  - GENBANK/AJ578126
SI  - GENBANK/AJ578127
SI  - GENBANK/AJ578128
SI  - GENBANK/AJ578129
SI  - GENBANK/AJ578130
SI  - GENBANK/AJ578131
SI  - GENBANK/AJ578132
SI  - GENBANK/AJ578133
SI  - GENBANK/AJ578134
SI  - GENBANK/AJ578135
SI  - GENBANK/AJ578136
SI  - GENBANK/AJ578137
SI  - GENBANK/AJ578138
SI  - GENBANK/AJ578139
SI  - GENBANK/AJ578140
SI  - GENBANK/AJ578141
SI  - GENBANK/AJ578142
SI  - GENBANK/AJ578143
SI  - GENBANK/AJ578144
SI  - GENBANK/AJ578145
SI  - GENBANK/AJ578146
SI  - GENBANK/AJ578147
SI  - GENBANK/AJ578148
SI  - GENBANK/AJ578149
SI  - GENBANK/AJ578150
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Appl Environ Microbiol
JT  - Applied and environmental microbiology
JID - 7605801
RN  - 0 (DNA, Archaeal)
RN  - 0 (DNA, Ribosomal)
RN  - 0 (RNA, Ribosomal, 16S)
RN  - OP0UW79H66 (Methane)
SB  - IM
MH  - Archaea/*classification/genetics/*isolation & purification/metabolism
MH  - *Cold Temperature
MH  - DNA, Archaeal/analysis
MH  - DNA, Ribosomal/analysis
MH  - Geologic Sediments/*microbiology
MH  - In Situ Hybridization, Fluorescence
MH  - Methane/*metabolism
MH  - Molecular Sequence Data
MH  - Oregon
MH  - Phylogeny
MH  - RNA, Ribosomal, 16S/genetics
MH  - Seawater/*microbiology
MH  - Sequence Analysis, DNA
MH  - Thiotrichaceae/growth & development
MH  - Ukraine
PMC - PMC544223
EDAT- 2005/01/11 09:00
MHDA- 2005/03/16 09:00
PMCR- 2005/01/01
CRDT- 2005/01/11 09:00
PHST- 2005/01/11 09:00 [pubmed]
PHST- 2005/03/16 09:00 [medline]
PHST- 2005/01/11 09:00 [entrez]
PHST- 2005/01/01 00:00 [pmc-release]
AID - 71/1/467 [pii]
AID - 0814-04 [pii]
AID - 10.1128/AEM.71.1.467-479.2005 [doi]
PST - ppublish
SO  - Appl Environ Microbiol. 2005 Jan;71(1):467-79. doi: 
      10.1128/AEM.71.1.467-479.2005.