PMID- 15640395
OWN - NLM
STAT- MEDLINE
DCOM- 20050818
LR  - 20131121
IS  - 8750-7587 (Print)
IS  - 0161-7567 (Linking)
VI  - 98
IP  - 5
DP  - 2005 May
TI  - Leptin gene expression and systemic levels in healthy men: effect of exercise, 
      carbohydrate, interleukin-6, and epinephrine.
PG  - 1805-12
AB  - Leptin, an adipose tissue-derived cytokine, is correlated with adipose mass as 
      obese persons have increased levels of leptin that decrease with weight loss. 
      Previous studies demonstrate that high-energy-expenditure exercise decreases 
      circulating leptin levels, whereas low-energy-expenditure exercise has no effect. 
      We aimed to test the hypothesis that acute exercise reduced leptin mRNA levels in 
      human adipose tissue and that this effect would be ameliorated by carbohydrate 
      supplementation. Because exercise markedly increases circulating IL-6 and 
      epinephrine, we investigated whether the changes in leptin seen with acute 
      exercise could be mediated by IL-6 or epinephrine infusion. Abdominal 
      subcutaneous adipose tissue mRNA and plasma levels of leptin were measured in 
      healthy men in response to 3-h ergometer exercise with or without carbohydrate 
      (CHO) ingestion (n = 8) and in response to infusion with recombinant human 
      (rh)IL-6 (n = 11) or epinephrine (n = 8) or saline. Plasma leptin declined in 
      response to exercise (P < 0.05) compared with rest, whereas mRNA expression in 
      adipose tissue was unaffected. The exercise-induced decrease in plasma leptin was 
      attenuated by CHO ingestion (P < 0.001). A 3-h epinephrine infusion decreased 
      plasma leptin (P < 0.001) to the same level seen with 3 h of exercise, whereas 
      leptin levels were unaffected by rhIL-6 infusion. In conclusion, both acute 
      exercise and epinephrine infusion decreased plasma leptin to a similar extent, 
      whereas there was no effect with rhIL-6 infusion. Acute exercise solely affected 
      leptin plasma levels, as mRNA levels were unchanged. The exercise-induced 
      decrease in circulating leptin was counteracted by CHO ingestion, suggesting a 
      posttranscriptional regulatory mechanism of leptin involving substrate 
      availability.
FAU - Keller, Pernille
AU  - Keller P
AD  - Centre of Inflammation and Metabolism, Department of Infectious Diseases and The 
      Copenhagen Muscle Research Center, Faculty of Health Sciences, University 
      Hospital of Copenhagen, Denmark.
FAU - Keller, Charlotte
AU  - Keller C
FAU - Steensberg, Adam
AU  - Steensberg A
FAU - Robinson, Lindsay E
AU  - Robinson LE
FAU - Pedersen, Bente K
AU  - Pedersen BK
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20050107
PL  - United States
TA  - J Appl Physiol (1985)
JT  - Journal of applied physiology (Bethesda, Md. : 1985)
JID - 8502536
RN  - 0 (Dietary Carbohydrates)
RN  - 0 (Interleukin-6)
RN  - 0 (Leptin)
RN  - 0 (RNA, Messenger)
RN  - 0 (Recombinant Proteins)
RN  - YKH834O4BH (Epinephrine)
SB  - IM
MH  - Adipose Tissue/drug effects/metabolism
MH  - Adult
MH  - Dietary Carbohydrates/*blood
MH  - Epinephrine/*pharmacology
MH  - Exercise/*physiology
MH  - Gene Expression Regulation/drug effects/physiology
MH  - Humans
MH  - Interleukin-6/administration & dosage/*pharmacology
MH  - Leptin/*biosynthesis/blood/*genetics
MH  - Male
MH  - RNA, Messenger/biosynthesis/genetics
MH  - Recombinant Proteins/administration & dosage/pharmacology
EDAT- 2005/01/11 09:00
MHDA- 2005/08/19 09:00
CRDT- 2005/01/11 09:00
PHST- 2005/01/11 09:00 [pubmed]
PHST- 2005/08/19 09:00 [medline]
PHST- 2005/01/11 09:00 [entrez]
AID - 00592.2004 [pii]
AID - 10.1152/japplphysiol.00592.2004 [doi]
PST - ppublish
SO  - J Appl Physiol (1985). 2005 May;98(5):1805-12. doi: 
      10.1152/japplphysiol.00592.2004. Epub 2005 Jan 7.