PMID- 15643009
OWN - NLM
STAT- MEDLINE
DCOM- 20050616
LR  - 20181113
IS  - 1071-412X (Print)
IS  - 1098-6588 (Electronic)
IS  - 1071-412X (Linking)
VI  - 12
IP  - 1
DP  - 2005 Jan
TI  - Differentiation, maturation, and survival of dendritic cells by osteopontin 
      regulation.
PG  - 206-12
AB  - Dendritic cells (DCs) are antigen-presenting cells with the ability to induce 
      primary immune responses necessary in innate immunity and adaptive immunity. 
      Osteopontin (OPN) is a secreted acidic phosphoprotein containing an 
      arginine-glycine-aspartate sequence and has been suggested to play an important 
      role in early cellular immune responses. The interaction between DCs and OPN has 
      not been clarified. We hypothesized that there is an important interaction 
      between DCs and OPN, which is an indispensable extracellular matrix component in 
      early cellular immune responses. Human monocyte-derived DCs synthesized OPN 
      especially during the differentiation from monocytes to immature DCs. By blocking 
      of OPN with anti-OPN antibody, cultured DCs became smaller and expressed lower 
      levels of costimulatory molecules and major histocompatibility complex class II 
      antigens than untreated DCs. Furthermore, DCs treated with anti-OPN antibody 
      easily underwent apoptosis. These results suggest that human DCs can produce OPN 
      and that OPN may play a role in the differentiation, maturation, and survival of 
      DCs by autocrine and/or paracrine pathways.
FAU - Kawamura, Kodai
AU  - Kawamura K
AD  - Department of Respiratory Medicine, Graduate School of Medical Sciences, Kumamoto 
      University, 1-1-1 Honjo, Kumamoto 860-8556, Japan.
FAU - Iyonaga, Kazuhiro
AU  - Iyonaga K
FAU - Ichiyasu, Hidenori
AU  - Ichiyasu H
FAU - Nagano, Junji
AU  - Nagano J
FAU - Suga, Moritaka
AU  - Suga M
FAU - Sasaki, Yutaka
AU  - Sasaki Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Clin Diagn Lab Immunol
JT  - Clinical and diagnostic laboratory immunology
JID - 9421292
RN  - 0 (SPP1 protein, human)
RN  - 0 (Sialoglycoproteins)
RN  - 106441-73-0 (Osteopontin)
SB  - IM
MH  - Apoptosis/physiology
MH  - Cell Differentiation/*physiology
MH  - Cell Survival/physiology
MH  - Dendritic Cells/*cytology/*metabolism
MH  - Flow Cytometry
MH  - Humans
MH  - Monocytes/cytology/metabolism
MH  - Osteopontin
MH  - Sialoglycoproteins/*metabolism
MH  - Stem Cells/cytology/metabolism
PMC - PMC540203
EDAT- 2005/01/12 09:00
MHDA- 2005/06/17 09:00
PMCR- 2005/01/01
CRDT- 2005/01/12 09:00
PHST- 2005/01/12 09:00 [pubmed]
PHST- 2005/06/17 09:00 [medline]
PHST- 2005/01/12 09:00 [entrez]
PHST- 2005/01/01 00:00 [pmc-release]
AID - 12/1/206 [pii]
AID - 0172-04 [pii]
AID - 10.1128/CDLI.12.1.206-212.2005 [doi]
PST - ppublish
SO  - Clin Diagn Lab Immunol. 2005 Jan;12(1):206-12. doi: 
      10.1128/CDLI.12.1.206-212.2005.