PMID- 15643549
OWN - NLM
STAT- MEDLINE
DCOM- 20050330
LR  - 20151119
IS  - 0032-0943 (Print)
IS  - 0032-0943 (Linking)
VI  - 70
IP  - 12
DP  - 2004 Dec
TI  - Inhibitory effects of Centella asiatica water extract and asiaticoside on 
      inducible nitric oxide synthase during gastric ulcer healing in rats.
PG  - 1150-4
AB  - In this study, the effects of Centella asiatica water extract (CE) and its active 
      constituent, asiaticoside (AC), on the expression and activity of inducible 
      nitric oxide synthase (iNOS) during gastric ulcer healing in rats were 
      investigated. CE was prepared from Centella asiatica dry plant and the 
      concentration of AC in CE was quantitatively determined with the use of high 
      performance liquid chromatography analysis. Different concentrations of CE (0.10 
      g/kg and 0.25 g/kg) and AC (5 mg/kg and 10 mg/kg) were orally administered to 
      rats with acetic acid-induced gastric ulcers. They were found to reduce the size 
      of the ulcers at days 1, 3 and 7 after ulcer induction in a dose-dependent 
      manner, with a concomitant attenuation of iNOS activity and protein expression at 
      the ulcer tissues. The levels of nitrite and nitrate (NO(X)-), the stable 
      end-products of nitric oxide (NO), in the gastric ulcer tissues were also 
      decreased. N-[3-(aminomethyl)benzyl]acetamidine (1400W), a highly selective 
      inhibitor of iNOS, was found to produce similar but more potent inhibition on 
      iNOS activity at a dose of 0.1 mg/kg. These findings indicate that CE and AC have 
      an anti-inflammatory property that is brought about by inhibition of NO synthesis 
      and thus facilitates ulcer healing.
FAU - Guo, Jin Sheng
AU  - Guo JS
AD  - Department of Pharmacology, Faculty of Medicine, The University of Hong Kong, 
      Hong Kong.
FAU - Cheng, Chuen Lung
AU  - Cheng CL
FAU - Koo, Marcel Wing Leung
AU  - Koo MW
LA  - eng
PT  - Journal Article
PL  - Germany
TA  - Planta Med
JT  - Planta medica
JID - 0066751
RN  - 0 (Anti-Ulcer Agents)
RN  - 0 (Enzyme Inhibitors)
RN  - 0 (Plant Extracts)
RN  - 0 (Triterpenes)
RN  - EC 1.14.13.39 (Nitric Oxide Synthase)
RN  - EC 1.14.13.39 (Nitric Oxide Synthase Type II)
RN  - EC 1.14.13.39 (Nos2 protein, rat)
RN  - PKO39VY215 (asiaticoside)
SB  - IM
MH  - Animals
MH  - Anti-Ulcer Agents/administration & dosage/*pharmacology/therapeutic use
MH  - *Centella
MH  - Dose-Response Relationship, Drug
MH  - Enzyme Inhibitors/administration & dosage/pharmacology/therapeutic use
MH  - Male
MH  - Nitric Oxide Synthase/antagonists & inhibitors/*biosynthesis/*drug effects
MH  - Nitric Oxide Synthase Type II
MH  - *Phytotherapy
MH  - Plant Extracts/administration & dosage/*pharmacology/therapeutic use
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Stomach Ulcer/drug therapy/enzymology/pathology
MH  - Triterpenes/administration & dosage/*pharmacology/therapeutic use
EDAT- 2005/01/12 09:00
MHDA- 2005/03/31 09:00
CRDT- 2005/01/12 09:00
PHST- 2005/01/12 09:00 [pubmed]
PHST- 2005/03/31 09:00 [medline]
PHST- 2005/01/12 09:00 [entrez]
AID - 10.1055/s-2004-835843 [doi]
PST - ppublish
SO  - Planta Med. 2004 Dec;70(12):1150-4. doi: 10.1055/s-2004-835843.