PMID- 15643650
OWN - NLM
STAT- MEDLINE
DCOM- 20050418
LR  - 20061115
IS  - 1076-5174 (Print)
IS  - 1076-5174 (Linking)
VI  - 40
IP  - 1
DP  - 2005 Jan
TI  - Glycan side chains on naturally presented MHC class II ligands.
PG  - 100-4
AB  - The molecular characterization of unknown naturally presented major 
      histocompatibility complex (MHC) class II glycopeptides carrying complex glycans 
      has so far not been achieved, reflecting the different fragmentation 
      characteristics of sugars and peptides in mass spectrometric analysis. Human 
      leukocyte antigen (HLA)-DR-bound peptides were isolated by affinity purification, 
      separated via high performance liquid chromatography and analyzed by 
      matrix-assisted laser desorption/ionization and electrospray ionization mass 
      spectrometry. We were able to identify two naturally processed MHC class II 
      ligands, CD53(122-136) and CD53(121-136), carrying complex N-linked glycan side 
      chains by a combination of in-source and collision-induced fragmentation on a 
      quadrupole time-of-flight tandem mass spectrometer.
FAU - Dengjel, Jorn
AU  - Dengjel J
AD  - Department of Immunology, Institute for Cell Biology, University of Tubingen, 
      72076 Tubingen, Germany.
FAU - Rammensee, Hans-Georg
AU  - Rammensee HG
FAU - Stevanovic, Stefan
AU  - Stevanovic S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Mass Spectrom
JT  - Journal of mass spectrometry : JMS
JID - 9504818
RN  - 0 (HLA Antigens)
RN  - 0 (Histocompatibility Antigens Class II)
RN  - 0 (Ligands)
RN  - 0 (Polysaccharides)
SB  - IM
MH  - Glycosylation
MH  - HLA Antigens/*analysis
MH  - Histocompatibility Antigens Class II/*analysis
MH  - Humans
MH  - Ligands
MH  - Polysaccharides/*analysis
MH  - Spectrometry, Mass, Electrospray Ionization/*methods
MH  - Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization/*methods
EDAT- 2005/01/12 09:00
MHDA- 2005/04/19 09:00
CRDT- 2005/01/12 09:00
PHST- 2005/01/12 09:00 [pubmed]
PHST- 2005/04/19 09:00 [medline]
PHST- 2005/01/12 09:00 [entrez]
AID - 10.1002/jms.780 [doi]
PST - ppublish
SO  - J Mass Spectrom. 2005 Jan;40(1):100-4. doi: 10.1002/jms.780.