PMID- 1564448
OWN - NLM
STAT- MEDLINE
DCOM- 19920521
LR  - 20190827
IS  - 0146-6615 (Print)
IS  - 0146-6615 (Linking)
VI  - 36
IP  - 3
DP  - 1992 Mar
TI  - Lack of detectable human T-cell lymphotropic virus type I sequences in samples 
      from multiple sclerosis patients.
PG  - 155-61
AB  - Recently, inconclusive results have followed the early data on the possible 
      association between multiple sclerosis (MS) and human T-cell lymphotropic virus 
      type I (HTLV-I) infection. For this reason, we examined this hypothesis using the 
      polymerase chain reaction (PCR) to study samples of differing origin from Italian 
      MS patients. In particular, we developed a systematic analysis of 
      paraffin-embedded brain white matter from histologically defined lesions of 14 MS 
      patients using PCR and primer sets specific for HTLV-I sequences; additionally, 
      cerebrospinal fluids (CSFs) from 12 patients and peripheral blood mononuclear 
      cells (PBMCs) from subjects at the early and late phase of the disease were 
      investigated for free HTLV-I virions and specific proviral sequences, 
      respectively. In agreement with some groups who reported lack of HTLV-I sequences 
      in PBMCs of MS patients but in clear contrast with others, we failed to detect 
      specific viral sequences using this broad approach.
FAU - Menzo, S
AU  - Menzo S
AD  - Institute of Microbiology, University of Ancona, Italy.
FAU - Manzin, A
AU  - Manzin A
FAU - Bagnarelli, P
AU  - Bagnarelli P
FAU - Varaldo, P E
AU  - Varaldo PE
FAU - Grandi, G
AU  - Grandi G
FAU - Giuliani, G
AU  - Giuliani G
FAU - Cazzato, G
AU  - Cazzato G
FAU - Giacca, M
AU  - Giacca M
FAU - Clementi, M
AU  - Clementi M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Med Virol
JT  - Journal of medical virology
JID - 7705876
RN  - 0 (DNA, Viral)
SB  - IM
MH  - Base Sequence
MH  - Brain/microbiology
MH  - Cell Line
MH  - DNA, Viral/blood/cerebrospinal fluid/isolation & purification
MH  - Female
MH  - Human T-lymphotropic virus 1/*isolation & purification
MH  - Humans
MH  - Male
MH  - Middle Aged
MH  - Molecular Sequence Data
MH  - Multiple Sclerosis/*microbiology
MH  - Nucleic Acid Hybridization
MH  - Polymerase Chain Reaction
EDAT- 1992/03/01 00:00
MHDA- 1992/03/01 00:01
CRDT- 1992/03/01 00:00
PHST- 1992/03/01 00:00 [pubmed]
PHST- 1992/03/01 00:01 [medline]
PHST- 1992/03/01 00:00 [entrez]
AID - 10.1002/jmv.1890360302 [doi]
PST - ppublish
SO  - J Med Virol. 1992 Mar;36(3):155-61. doi: 10.1002/jmv.1890360302.