PMID- 1564545
OWN - NLM
STAT- MEDLINE
DCOM- 19920518
LR  - 20041117
IS  - 0022-3085 (Print)
IS  - 0022-3085 (Linking)
VI  - 76
IP  - 5
DP  - 1992 May
TI  - Suppression of anchorage-independent growth of human glioblastoma cell by major 
      histocompatibility complex class I gene-transfection.
PG  - 845-9
AB  - The host's immune system discriminates tumor cells from normal cells by 
      recognizing the major histocompatibility complex (MHC) class I antigen expressed 
      on the tumor cell membrane. However, the role of MHC class I antigen in tumor 
      cells has not yet been clarified. In this study, the influence of MHC class I 
      antigen expression on the tumorigenicity of a human glioblastoma cell line (KMG4) 
      is examined. Barely detectable levels of MHC class I messenger ribonucleic acid 
      were found to express in KMG4 cells by Northern blot analysis using mouse MHC 
      class I (H-2Ld) and human leukocyte antigen (HLA)-B7 genes as probes. The H-2Ld 
      gene connected at the downstream end of murine mammary tumor virus 
      (MMTV)-promoter was cotransfected with the neomycine-resistant gene pSV2-neo into 
      KMG4 cells, and the drug-resistant cells were selected. The KMG4 cells 
      (KMG4-MMTV-Ld), which acquired the MHC class I gene were detected by Northern 
      blot analysis with H-2Ld as the probe, and by immunohistochemistry using the 
      H-2Ld-specific monoclonal antibody. Tumorigenicity, as determined by 
      colony-forming ability in soft agar, was then compared between MHC class 
      I-expressing KMG4-MMTV-Ld and nonexpressing control cells. The MHC class 
      I-expressing cells were found to be deprived of colony-forming ability, 
      indicating that MHC class I antigen could negatively influence the 
      anchorage-independent cell growth of the human glioblastoma cell line KMG4.
FAU - Momozaki, N
AU  - Momozaki N
AD  - Department of Neurosurgery, Saga Medical School, Japan.
FAU - Oh-Uchida, M
AU  - Oh-Uchida M
FAU - Tabuchi, K
AU  - Tabuchi K
FAU - Ikezaki, K
AU  - Ikezaki K
FAU - Hori, K
AU  - Hori K
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Neurosurg
JT  - Journal of neurosurgery
JID - 0253357
RN  - 0 (H-2 Antigens)
SB  - IM
MH  - Animals
MH  - Blotting, Northern
MH  - Cell Division/genetics/immunology
MH  - Gene Expression
MH  - Genes, MHC Class I/*genetics
MH  - Glioma/*genetics/immunology/pathology
MH  - H-2 Antigens/genetics/physiology
MH  - Humans
MH  - Mice
MH  - Transfection
MH  - Tumor Cells, Cultured
EDAT- 1992/05/01 00:00
MHDA- 1992/05/01 00:01
CRDT- 1992/05/01 00:00
PHST- 1992/05/01 00:00 [pubmed]
PHST- 1992/05/01 00:01 [medline]
PHST- 1992/05/01 00:00 [entrez]
AID - 10.3171/jns.1992.76.5.0845 [doi]
PST - ppublish
SO  - J Neurosurg. 1992 May;76(5):845-9. doi: 10.3171/jns.1992.76.5.0845.