PMID- 15645975
OWN - NLM
STAT- MEDLINE
DCOM- 20050210
LR  - 20220311
IS  - 0004-5772 (Print)
IS  - 0004-5772 (Linking)
VI  - 52
DP  - 2004 Jul
TI  - Anti-neutrophil cytoplasmic antibodies (ANCA) in systemic lupus erythematosus: 
      prevalence, clinical associations and correlation with other autoantibodies.
PG  - 533-7
AB  - AIM: This study was undertaken to clarify the nature of anti-neutrophil 
      cytoplasmic antibodies (ANCA) along with other autoantibodies in lupus nephritis 
      (LN) patients and in systemic lupus erythematosus (SLE) patients without 
      nephritis and to know their correlation with clinical manifestations and presence 
      of other autoantibodies. MATERIAL AND METHODS: Fourty one LN patients and 18 SLE 
      patients without nephritis were studied. LN patients were subdivided into diffuse 
      proliferative glomerulonephritis (DPGN), focal proliferative glomerulonephritis 
      (FPGN), rapidly progressive glomerulonephritis (RPGN) and membranoproliferative 
      glomerulonephritis (MPGN). Anti-neutrophil cytoplasmic antibodies (ANCA) were 
      detected by indirect immunofluorescence and confocal laser scanning microscope 
      using PMN and HL60 cells. ANCA specificities like anti-myeloperoxidase 
      (anti-MPO), anti-proteinase 3 (anti-PR3), anti-lactoferrin (anti-LF) and 
      anti-cathepsin G (anti-CG) were detected by ELISA. Other autoantibodies like 
      anti-nuclear antibodies (ANA), anti-double stranded DNA (anti-dsDNA), anti-single 
      stranded DNA(anti-ssDNA), anti-ribonucleoproteins (anti-nRNP), anti-Smith 
      antibodies (anti-Sm) and rheumatoid factor (RF) were also tested. RESULTS: ANCA 
      was detected in 37.3% patients. The predominant ANCA pattern was perinuclear 
      (p-ANCA). ANCA positivity was higher in LN patients and when confirmed by ELISA, 
      54.5% ANCA positives had anti-myeloperoxidase (anti-MPO). The cytoplasmic ANCA 
      (c-ANCA) pattern was not seen in any patient. Two patients having FPGN with 
      crescents showed atypical 'X-ANCA' pattern with dual specificity to anti-MPO and 
      anti-PR3 by ELISA. The titers of ANCA were more in LN as compared to SLE without 
      nephritis. LN cases having DPGN, FPGN, RPGN with crescents had higher titer 
      p-ANCA positivity with corresponding anti-MPO antibodies, along with ANA, 
      anti-dsDNA, anti-ssDNA and anti-Sm + anti-nRNP and also high SLEDAI scores. 
      CONCLUSION: ANCA in SLE may be used as a serological marker along with clinical 
      and histopathological assessment to differentiate vasculitides in LN cases from 
      SLE without nephritis.
FAU - Pradhan, Vandana D
AU  - Pradhan VD
AD  - Department of Autoimmune disorders, Institute of Immunohaematology, Indian 
      Council of Medical Research, 13th floor, KEM Hospital, Mumbai 400 012.
FAU - Badakere, S S
AU  - Badakere SS
FAU - Bichile, Lata S
AU  - Bichile LS
FAU - Almeida, A F
AU  - Almeida AF
LA  - eng
PT  - Journal Article
PL  - India
TA  - J Assoc Physicians India
JT  - The Journal of the Association of Physicians of India
JID - 7505585
RN  - 0 (Antibodies, Antineutrophil Cytoplasmic)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Antibodies, Antineutrophil Cytoplasmic/*blood
MH  - Child
MH  - Diagnosis, Differential
MH  - Female
MH  - Humans
MH  - Lupus Erythematosus, Systemic/blood/*diagnosis/immunology
MH  - Lupus Nephritis/blood/*diagnosis/immunology
MH  - Male
MH  - Middle Aged
EDAT- 2005/01/14 09:00
MHDA- 2005/02/11 09:00
CRDT- 2005/01/14 09:00
PHST- 2005/01/14 09:00 [pubmed]
PHST- 2005/02/11 09:00 [medline]
PHST- 2005/01/14 09:00 [entrez]
PST - ppublish
SO  - J Assoc Physicians India. 2004 Jul;52:533-7.