PMID- 15648285
OWN - NLM
STAT- MEDLINE
DCOM- 20050418
LR  - 20201209
IS  - 1726-4901 (Print)
IS  - 1726-4901 (Linking)
VI  - 67
IP  - 10
DP  - 2004 Oct
TI  - Immunohistochemical expression of Wilms' tumor 1 protein in nephroblastoma.
PG  - 506-10
AB  - BACKGROUND: Approximately 10% of nephroblastomas (Wilms' tumors) carry mutations 
      in the Wilms' tumor 1 (WT1) gene. Recently, a WT1 antibody raised against 
      N-terminal 1-181 amino acids of human WT1 became commercially available for 
      immunohistochemical use on paraffin-embedded tissue. The aim of this study was to 
      investigate the diagnostic and prognostic value of WT1 N-terminal antibody in 
      nephroblastomas. METHODS: Twenty-five patients with nephroblastoma were studied. 
      Four clear cell sarcomas of the kidney (CCSK) and 15 neuroblastomas were included 
      for comparative study. WT1 immunostaining was performed on paraffin material 
      using the WT1(6F-H2) antibody. The patients were staged according to the National 
      Wilms' Tumor Study (NWTS) staging system. RESULTS: Eleven tumors (44%) showed 
      blastemal nuclear staining with or without epithelial nuclear staining. Three of 
      the 13 low-stage tumors (stages I and II) showed WT1 blastemal nuclear staining, 
      while 8 of the 12 high stage (stage III and IV) tumors revealed blastemal nuclear 
      staining. The blastemal nuclear expressions of WT1 were statistically 
      significantly correlated with clinical stage (p = 0.036). All the neuroblastomas 
      and CCSK showed no nuclear immunoreactivity. CONCLUSIONS: The presence of WT1 
      nuclear immunoreactivity may be helpful to distinguish blastemal predominant 
      nephroblastomas from CCSK and neuroblastomas.
FAU - Chen, Be-Fong
AU  - Chen BF
AD  - Department of Pathology, Mackay Memorial Hospital, Taipei, Taiwan, ROC. 
      a5514@ms2.mmh.org.tw
FAU - Tzen, Chin-yuen
AU  - Tzen CY
FAU - Liang, Der-Cherng
AU  - Liang DC
FAU - Liu, Hsi-Che
AU  - Liu HC
FAU - Huang, Yu-Wen
AU  - Huang YW
FAU - Fan, Chi-Chen
AU  - Fan CC
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - J Chin Med Assoc
JT  - Journal of the Chinese Medical Association : JCMA
JID - 101174817
RN  - 0 (Carrier Proteins)
RN  - 0 (Cell Cycle Proteins)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Nuclear Proteins)
RN  - 0 (RNA Splicing Factors)
RN  - 0 (WTAP protein, human)
SB  - IM
MH  - Adolescent
MH  - Carrier Proteins/*analysis
MH  - Cell Cycle Proteins
MH  - Child
MH  - Child, Preschool
MH  - DNA-Binding Proteins/*analysis
MH  - Female
MH  - Humans
MH  - Immunohistochemistry
MH  - Infant
MH  - Inpatients
MH  - Male
MH  - Nuclear Proteins/*analysis
MH  - Prognosis
MH  - RNA Splicing Factors
MH  - Taiwan
MH  - Wilms Tumor/metabolism/*pathology
EDAT- 2005/01/15 09:00
MHDA- 2005/04/19 09:00
CRDT- 2005/01/15 09:00
PHST- 2005/01/15 09:00 [pubmed]
PHST- 2005/04/19 09:00 [medline]
PHST- 2005/01/15 09:00 [entrez]
PST - ppublish
SO  - J Chin Med Assoc. 2004 Oct;67(10):506-10.