PMID- 15648549
OWN - NLM
STAT- MEDLINE
DCOM- 20050426
LR  - 20181113
IS  - 0391-4097 (Print)
IS  - 0391-4097 (Linking)
VI  - 27
IP  - 9
DP  - 2004 Oct
TI  - Intracerebroventricular administration of atrial natriuretic peptide prevents 
      increase of plasma ADH, aldosterone and corticosterone levels in restrained 
      conscious dehydrated rabbits.
PG  - 844-53
AB  - In order to investigate the effects of centrally administered ANP on plasma ADH, 
      aldosterone and corticosterone levels as well as on blood pressure and on heart 
      rate, 20 male New Zealand White (NZW) rabbits were used. Measurements were made 
      on restrained conscious animals one week after the implantation of an indwelling 
      intracerebroventricular (icv) cannula and two indwelling intravascular catheters 
      (intracarotid and intrajugular). Animals were classified into two main groups, 
      those with water available ad libitum ("euhydrated" group) and those who were 
      dehydrated for 24 h ("dehydrated" group) before blood pressure and heart rate 
      recordings and blood sampling for hormonal determination. Each group's 
      individuals were divided into two subgroups of five animals each. Blood samples 
      were collected at 0 min (control) and 30, 60, 90, 120 min following icv 
      administration of 25 microl of either artificial cerebrospinal fluid (aCSF) 
      (subgroups "aCSF") or human (h) ANP (1 microg) in aCSF (25 microl) (subgroups 
      "hANP"). Blood pressure and heart rate were also recorded at the same times. 
      Plasma ADH, aldosterone and corticosterone concentrations were determined using 
      RIA. The results were analysed by analysis of variance (ANOVA). Blood pressure 
      and heart rate values were unaffected by water deprivation or by ANP 
      administration. Mean plasma corticosterone levels at all times (30-120 min) were 
      significantly higher (p<0.001) than those at 0 min time. Plasma corticosterone 
      levels in the "dehydrated+aCSF" group were significantly higher (p<0.05) than in 
      each of the other groups ("dehydrated+hANP", "euhydrated+aCSF", 
      "euhydrated+hANP"). Plasma corticosterone levels in each of those other groups 
      did not differ significantly from one another. Dehydration resulted in a tendency 
      to increase in aldosterone levels (p<0.07), and icv administration of hANP tended 
      (p<0.08) to prevent in the "dehydrated+hANP" experimental group the increase in 
      aldosterone levels observed in the control "dehydrated+aCSF" group from 30 to 120 
      min. Dehydration resulted in an increase in ADH levels (p<0.0001), and icv 
      administration of hANP prevented (p<0.05) in "dehydrated+hANP" experimental group 
      the increase in ADH levels observed in the control "dehydrated+aCSF" group from 
      90 to 120 min. The increase of corticosterone and ADH and the tendency towards 
      increase in aldosterone in the control dehydrated groups could possibly be due to 
      the combined stress stimulus of dehydration and restriction in the restrain box. 
      These results indicate that centrally administered ANP, at the concentration 
      achieved in the present study, neither affects blood pressure and heart rate in 
      conscious restrained euhydrated and 24 h-dehydrated NZW rabbits nor decreases the 
      ADH, aldosterone and corticosterone response to dehydration, but does apparently 
      modulate ADH, aldosterone and corticosterone responses to other stimuli in the 
      dehydrated state. In conclusion, the results of this study confirm that brain ANP 
      may have an inhibitory effect on stimulated ADH, aldosterone and corticosterone 
      release.
FAU - Kallaras, C
AU  - Kallaras C
AD  - Department of Experimental Physiology, Faculty of Medicine, Aristotle University 
      of Thessaloniki, Thessaloniki, Greece. kallaras@med.auth.gr
FAU - Angelopoulos, N
AU  - Angelopoulos N
FAU - Bountzioukas, S
AU  - Bountzioukas S
FAU - Mavroudis, K
AU  - Mavroudis K
FAU - Karamouzis, M
AU  - Karamouzis M
FAU - Guiba-Tziampiri, O
AU  - Guiba-Tziampiri O
LA  - eng
PT  - Journal Article
PL  - Italy
TA  - J Endocrinol Invest
JT  - Journal of endocrinological investigation
JID - 7806594
RN  - 11000-17-2 (Vasopressins)
RN  - 4964P6T9RB (Aldosterone)
RN  - 85637-73-6 (Atrial Natriuretic Factor)
RN  - W980KJ009P (Corticosterone)
SB  - IM
MH  - Aldosterone/*blood
MH  - Animals
MH  - Atrial Natriuretic Factor/*administration & dosage/pharmacology
MH  - Blood Pressure/drug effects
MH  - Corticosterone/*blood
MH  - Dehydration/*blood
MH  - Heart Rate/drug effects
MH  - Humans
MH  - Injections, Intraventricular
MH  - Male
MH  - Osmolar Concentration
MH  - Rabbits
MH  - *Restraint, Physical
MH  - Vasopressins/*blood
EDAT- 2005/01/15 09:00
MHDA- 2005/04/27 09:00
CRDT- 2005/01/15 09:00
PHST- 2005/01/15 09:00 [pubmed]
PHST- 2005/04/27 09:00 [medline]
PHST- 2005/01/15 09:00 [entrez]
AID - 5191 [pii]
AID - 10.1007/BF03346279 [doi]
PST - ppublish
SO  - J Endocrinol Invest. 2004 Oct;27(9):844-53. doi: 10.1007/BF03346279.