PMID- 15648567
OWN - NLM
STAT- MEDLINE
DCOM- 20050630
LR  - 20190922
IS  - 0920-5063 (Print)
IS  - 0920-5063 (Linking)
VI  - 15
IP  - 11
DP  - 2004
TI  - Controlled release of plasmid DNA from biodegradable scaffolds fabricated using a 
      thermally-induced phase-separation method.
PG  - 1341-53
AB  - Highly porous poly(D,L-lactic-co-glycolic acid) (PLGA) scaffolds were fabricated 
      by a thermally-induced phase-separation (TIPS) method to deliver plasmid DNA in a 
      controlled manner. A variety of TIPS parameters directly affecting pore 
      structures and their interconnectivities of the scaffold, such as polymer 
      concentration, solvent/non-solvent ratio, quenching methods and annealing time, 
      were systematically examined to explore their effects on sustained release 
      behaviors of plasmid DNA. Plasmid DNA was directly loaded into the inner pore 
      region of the scaffold during the TIPS process. By optimizing the parameters, 
      PLGA scaffolds releasing plasmid DNA over 21 days were successfully fabricated. 
      DNA release profiles were mainly affected by the pore structures and their 
      interconnectivities of the scaffolds. Plasmid DNA released from the scaffolds 
      fully maintained its structural integrity and showed comparable transfection 
      efficiency to native plasmid DNA. These biodegradable polymeric scaffolds capable 
      of sustained DNA release can be potentially applied for various tissue 
      engineering purposes requiring a combined gene delivery strategy.
FAU - Chun, Ki Woo
AU  - Chun KW
AD  - Department of Biological Sciences, Korea Advanced Institute of Science and 
      Technology, Daejeon 305-701, South Korea.
FAU - Cho, Kyung Chul
AU  - Cho KC
FAU - Kim, Sun Hwa
AU  - Kim SH
FAU - Jeong, Ji Hoon
AU  - Jeong JH
FAU - Park, Tae Gwan
AU  - Park TG
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Biomater Sci Polym Ed
JT  - Journal of biomaterials science. Polymer edition
JID - 9007393
RN  - 0 (Polymers)
RN  - 0 (Solvents)
RN  - 1SIA8062RS (Polylactic Acid-Polyglycolic Acid Copolymer)
RN  - 26009-03-0 (Polyglycolic Acid)
RN  - 33X04XA5AT (Lactic Acid)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - *Absorbable Implants
MH  - Cell Line
MH  - DNA/chemistry/*metabolism
MH  - Humans
MH  - Lactic Acid/*chemistry/*metabolism
MH  - Microscopy, Electron, Scanning
MH  - Plasmids/chemistry/*metabolism
MH  - Polyglycolic Acid/*chemistry/*metabolism
MH  - Polylactic Acid-Polyglycolic Acid Copolymer
MH  - Polymers/*chemistry/*metabolism
MH  - Solvents
MH  - *Temperature
MH  - Transfection
EDAT- 2005/01/15 09:00
MHDA- 2005/07/01 09:00
CRDT- 2005/01/15 09:00
PHST- 2005/01/15 09:00 [pubmed]
PHST- 2005/07/01 09:00 [medline]
PHST- 2005/01/15 09:00 [entrez]
AID - 10.1163/1568562042368103 [doi]
PST - ppublish
SO  - J Biomater Sci Polym Ed. 2004;15(11):1341-53. doi: 10.1163/1568562042368103.