PMID- 15648785
OWN - NLM
STAT- MEDLINE
DCOM- 20050428
LR  - 20131121
IS  - 0091-7370 (Print)
IS  - 0091-7370 (Linking)
VI  - 34
IP  - 4
DP  - 2004 Autumn
TI  - Pentoxifylline inhibits endotoxin-induced NF-kappa B activation and associated 
      production of proinflammatory cytokines.
PG  - 427-36
AB  - The effects of graded doses of pentoxifylline (PTX) on endotoxin-induced 
      production of inflammatory cytokines and activation of nuclear factor kappa B 
      (NF-kappaB) were studied in vivo in rat intestine. Sepsis was induced in rats by 
      ip injection of lipopolysaccharide (LPS, 5 mg/kg). PTX was injected via the tail 
      vein at dosages of 6.25, 12.5, 25, 50, or 100 mg/kg at 1 min after LPS challenge. 
      NF-kappaB activation in intestine was investigated by electrophoretic mobility 
      shift assay (EMSA). Tumor necrosis factor-alpha (TNF-alpha), interleukin-6 
      (IL-6), and interleukin-10 (IL-10) levels were measured in intestine by 
      enzyme-linked immunosorbance assays (ELISA). Intestinal TNF-alpha, IL-6, and 
      IL-10 mRNA expression were studied by the reverse-transcription polymerase chain 
      reaction (RT-PCR). The measurements of NF-kappaB, TNF-alpha, IL-6, and IL-10 were 
      performed, respectively, at 1, 4, 4, and 1 hr after endotoxin injection. The 
      results showed that LPS elevated the production of TNF-alpha, IL-6, and IL-10 and 
      enhanced NF-kappaB activation in rat intestine. At all dosages, PTX reduced the 
      activation of NF-kappaB and the production of TNF-alpha and IL-6, but enhanced 
      the release of IL-10. These effects were greatest at dosages of 50 mg/kg for 
      TNF-alpha and IL-6, and 25 mg/kg for IL-10. In conclusion, PTX suppressed the 
      production of proinflammatory cytokines such as TNF-alpha and IL-6 in rat 
      intestine, and enhanced the endotoxin-induced production of IL-10. The 
      suppressive effect of proinflammatory cytokines may act by inhibiting NF-kappaB 
      activation, but not by induction of IL-10.
FAU - Ji, Qing
AU  - Ji Q
AD  - Department of Anesthesiology, Jinling Hospital, Nanjing, People's Republic of 
      China.
FAU - Zhang, Lidong
AU  - Zhang L
FAU - Jia, Hongbin
AU  - Jia H
FAU - Xu, Jianguo
AU  - Xu J
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Ann Clin Lab Sci
JT  - Annals of clinical and laboratory science
JID - 0410247
RN  - 0 (Cytokines)
RN  - 0 (Interleukin-6)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (NF-kappa B)
RN  - 0 (RNA, Messenger)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 0 (Vasodilator Agents)
RN  - 130068-27-8 (Interleukin-10)
RN  - SD6QCT3TSU (Pentoxifylline)
SB  - IM
MH  - Animals
MH  - Cytokines/*biosynthesis/genetics
MH  - Disease Models, Animal
MH  - Dose-Response Relationship, Drug
MH  - Drug Therapy, Combination
MH  - Endotoxemia/*metabolism/pathology
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Injections, Intravenous
MH  - Interleukin-10/biosynthesis/genetics
MH  - Interleukin-6/biosynthesis/genetics
MH  - Jejunum/*drug effects/metabolism
MH  - Lipopolysaccharides/pharmacology
MH  - Male
MH  - NF-kappa B/*antagonists & inhibitors/biosynthesis
MH  - Pentoxifylline/administration & dosage/*pharmacology
MH  - RNA, Messenger/metabolism
MH  - Rats
MH  - Rats, Wistar
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Tumor Necrosis Factor-alpha/biosynthesis/genetics
MH  - Vasodilator Agents/administration & dosage/*pharmacology
EDAT- 2005/01/15 09:00
MHDA- 2005/04/29 09:00
CRDT- 2005/01/15 09:00
PHST- 2005/01/15 09:00 [pubmed]
PHST- 2005/04/29 09:00 [medline]
PHST- 2005/01/15 09:00 [entrez]
PST - ppublish
SO  - Ann Clin Lab Sci. 2004 Autumn;34(4):427-36.