PMID- 15648829
OWN - NLM
STAT- MEDLINE
DCOM- 20050323
LR  - 20131121
IS  - 0177-5103 (Print)
IS  - 0177-5103 (Linking)
VI  - 62
IP  - 1-2
DP  - 2004 Nov 23
TI  - Detection of Ostreid Herpesvirus 1 (OsHV-1) DNA in seawater by PCR: influence of 
      water parameters in bioassays.
PG  - 35-44
AB  - Since 1991, herpesvirus infections have been reported among larvae and juveniles 
      of various bivalves. Most of the studies focused on detection of viral infections 
      of economically important species. However, the persistence of bivalve 
      herpesviruses in the marine environment is poorly documented. The present study 
      concerns the role of seawater parameters in Ostreid Herpesvirus 1 (OsHV-1) 
      detection by polymerase chain reaction (PCR). Viral DNA extracted from purified 
      particles or virions present in infected oyster larvae were detected by PCR after 
      storage in different media at different temperatures. The lowest detection 
      threshold was found using distilled water or Tris EDTA buffer. In seawater, the 
      threshold was higher. The use of sterile media permitted detection of viral DNA 
      stored over a longer period. Storage temperature also had a significant influence 
      on detection, with lower temperatures promoting DNA detection over a longer 
      period. In summary, water parameters such as temperature influenced detection of 
      OsHV-1 DNA by PCR. However, the PCR technique may also be successfully applied to 
      samples in natural seawater. Indeed, the PCR technique permitted detection of 
      naked viral DNA at 100 ng l(-1) in seawater in bioassays.
FAU - Vigneron, Vassilia
AU  - Vigneron V
AD  - Laboratoire de Biologie et Environnement Marins, EA 3168, Universite de La 
      Rochelle, Avenue Michel Crepeau, 17042 La Rochelle, France.
FAU - Solliec, Gaelle
AU  - Solliec G
FAU - Montanie, Helene
AU  - Montanie H
FAU - Renault, Tristan
AU  - Renault T
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - Germany
TA  - Dis Aquat Organ
JT  - Diseases of aquatic organisms
JID - 8807037
RN  - 0 (DNA Primers)
RN  - 0 (DNA, Viral)
RN  - 9G34HU7RV0 (Edetic Acid)
SB  - IM
MH  - Animals
MH  - Biological Assay/methods
MH  - DNA Primers
MH  - DNA, Viral/*isolation & purification
MH  - Edetic Acid
MH  - Herpesviridae/*genetics
MH  - Kinetics
MH  - Larva/virology
MH  - Ostreidae/*virology
MH  - Polymerase Chain Reaction/*methods
MH  - Seawater/*virology
MH  - Temperature
MH  - Virion/isolation & purification
EDAT- 2005/01/15 09:00
MHDA- 2005/03/24 09:00
CRDT- 2005/01/15 09:00
PHST- 2005/01/15 09:00 [pubmed]
PHST- 2005/03/24 09:00 [medline]
PHST- 2005/01/15 09:00 [entrez]
AID - 10.3354/dao062035 [doi]
PST - ppublish
SO  - Dis Aquat Organ. 2004 Nov 23;62(1-2):35-44. doi: 10.3354/dao062035.