PMID- 15650119
OWN - NLM
STAT- MEDLINE
DCOM- 20050615
LR  - 20200930
IS  - 0363-6119 (Print)
IS  - 0363-6119 (Linking)
VI  - 288
IP  - 6
DP  - 2005 Jun
TI  - Regulation of expression of two aquaporin homologs in the intestine of the 
      European eel: effects of seawater acclimation and cortisol treatment.
PG  - R1733-43
AB  - Complementary DNAs encoding homologs of the mammalian aquaglyceroporins (termed 
      AQPe) and aquaporin-1 isoforms (termed AQP1) were isolated from the European eel. 
      The AQP amino acid sequences share 35-54% identity with other known human AQPs. 
      Although AQPe mRNA expression was approximately equivalent along the entire 
      length of the gut, AQP1 expression was the highest in the posterior/rectal 
      segment. Seawater (SW) acclimation increased AQP1 mRNA abundance by 5- and 
      17-fold in the anterior, 14- and 23-fold in the mid-, and 9- and 7-fold in the 
      posterior/rectal gut regions of yellow and silver eels, respectively. SW 
      acclimation had an effect on AQPe mRNA expression only in the midintestine of 
      silver eels, where a small but significant 1.7-fold increase in abundance was 
      measured. Western blots using an eel AQP1-specific antibody identified the 
      presence of a major immunoreactive 28-kDa protein, primarily within the 
      posterior/rectal segment. A 3-wk SW transfer induced an increase in AQP1 protein 
      abundance in all intestinal segments, with the posterior/rectal region still 
      expressing protein levels approximately 40- and 8-fold higher than the anterior 
      and midsegments, respectively. Strong AQP1 immunofluorescence was detected within 
      the vascular endothelium in both freshwater (FW)- and SW-acclimated eels and in 
      the epithelial apical brush border in the posterior/rectal gut regions of 
      SW-acclimated eels. Cortisol infusion into FW eels had no effect on intestinal 
      AQPe mRNA expression but induced increases in AQP1 mRNA and protein levels. These 
      results provide evidence for the presence of a SW-induced and steroid-regulated 
      AQP water channel pathway within the intestine of the European eel.
FAU - Martinez, Anne-Sophie
AU  - Martinez AS
AD  - School of Biology, Bute Medical Bldgs., University of St. Andrews, St. Andrews, 
      Fife, Scotland KY16 9TS, UK.
FAU - Cutler, Christopher P
AU  - Cutler CP
FAU - Wilson, Gillian D
AU  - Wilson GD
FAU - Phillips, Claire
AU  - Phillips C
FAU - Hazon, Neil
AU  - Hazon N
FAU - Cramb, Gordon
AU  - Cramb G
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20050113
PL  - United States
TA  - Am J Physiol Regul Integr Comp Physiol
JT  - American journal of physiology. Regulatory, integrative and comparative 
      physiology
JID - 100901230
RN  - 0 (Aquaporins)
RN  - 146410-94-8 (Aquaporin 1)
RN  - 63231-63-0 (RNA)
RN  - 9007-49-2 (DNA)
RN  - WI4X0X7BPJ (Hydrocortisone)
SB  - IM
MH  - Adaptation, Physiological/*physiology
MH  - Amino Acid Sequence
MH  - Animal Migration/physiology
MH  - Animals
MH  - Aquaporin 1
MH  - Aquaporins/*biosynthesis/genetics
MH  - Blotting, Northern
MH  - Cloning, Molecular
MH  - DNA/biosynthesis/genetics
MH  - Eels/*physiology
MH  - Gene Expression Regulation/drug effects
MH  - Hydrocortisone/administration & dosage/blood/*pharmacology
MH  - Intestinal Mucosa/metabolism
MH  - Intestines/*physiology
MH  - Microscopy, Fluorescence
MH  - Molecular Sequence Data
MH  - RNA/biosynthesis/isolation & purification
MH  - *Seawater
EDAT- 2005/01/15 09:00
MHDA- 2005/06/16 09:00
CRDT- 2005/01/15 09:00
PHST- 2005/01/15 09:00 [pubmed]
PHST- 2005/06/16 09:00 [medline]
PHST- 2005/01/15 09:00 [entrez]
AID - 00747.2004 [pii]
AID - 10.1152/ajpregu.00747.2004 [doi]
PST - ppublish
SO  - Am J Physiol Regul Integr Comp Physiol. 2005 Jun;288(6):R1733-43. doi: 
      10.1152/ajpregu.00747.2004. Epub 2005 Jan 13.