PMID- 15650589
OWN - NLM
STAT- MEDLINE
DCOM- 20050218
LR  - 20190722
IS  - 0017-9078 (Print)
IS  - 0017-9078 (Linking)
VI  - 88
IP  - 2
DP  - 2005 Feb
TI  - Electron paramagnetic resonance and fluorescence in situ hybridization-based 
      investigations of individual doses for persons living at Metlino in the upper 
      reaches of the Techa River.
PG  - 139-53
AB  - Waterborne releases to the Techa River from the Mayak Production Association in 
      Russia during 1949-1956 resulted in significant doses to persons living 
      downstream; the most contaminated village was Metlino, about 7 km from the site 
      of release. Internal and external doses have been estimated for these residents 
      using the Techa River Dosimetry System-2000 (TRDS-2000); the primary purpose is 
      to support epidemiological studies of the members of the Extended Techa River 
      Cohort. Efforts to validate the calculations of external and internal dose are 
      considered essential. One validation study of the TRDS-2000 system has been 
      performed by the comparison of calculated doses to quartz from bricks in old 
      buildings at Metlino with those measured by luminescence dosimetry. Two 
      additional methods of validation considered here are electron paramagnetic 
      resonance (EPR) measurements of teeth and fluorescence in situ hybridization 
      (FISH) measurements of chromosome translocations in circulating lymphocytes. For 
      electron paramagnetic resonance, 36 measurements on 26 teeth from 16 donors from 
      Metlino were made at the GSF-National Research Center for Environment and Health 
      (16 measurements) and the Institute of Metal Physics (20 measurements); the 
      correlation among measurements made at the two laboratories has been found to be 
      0.99. Background measurements were also made on 218 teeth (63 molars, 128 
      premolars, and 27 incisors). Fluorescence in situ hybridization measurements were 
      made for 31 residents of Metlino. These measurements were handicapped by the 
      analysis of a limited number of cells; for several individuals no stable 
      translocations were observed. Fluorescence in situ hybridization measurements 
      were also made for 39 individuals believed to be unexposed. The EPR- and 
      FISH-based estimates agreed well for permanent residents of Metlino: 0.67 +/- 
      0.21 Gy and 0.48 +/- 0.18 Gy (mean +/- standard error of the mean), respectively. 
      Results of the two experimental methods also agreed well with the estimates 
      derived from the use of the TRDS-2000. For all persons investigated according to 
      each technique, the EPR-measured dose to enamel was 0.55 +/- 0.17 Gy, and the 
      TRDS-2000 prediction for the dose to enamel for these individuals is 0.55 +/- 
      0.07 Gy. The fluorescence in situ hybridization-based dose, 0.38 +/- 0.10 Gy, 
      compared well to the TRDS-2000 prediction of external dose, 0.31 +/- 0.03 Gy, to 
      red bone marrow for these persons. Validation of external doses at the remaining 
      villages is an active area of investigation.
FAU - Degteva, Marina O
AU  - Degteva MO
AD  - Urals Research Center for Radiation Medicine, 454076 Chelyabinsk, Russia.
FAU - Anspaugh, Lynn R
AU  - Anspaugh LR
FAU - Akleyev, Alexander V
AU  - Akleyev AV
FAU - Jacob, Peter
AU  - Jacob P
FAU - Ivanov, Denis V
AU  - Ivanov DV
FAU - Wieser, Albrecht
AU  - Wieser A
FAU - Vorobiova, Marina I
AU  - Vorobiova MI
FAU - Shishkina, Elena A
AU  - Shishkina EA
FAU - Shved, Valentina A
AU  - Shved VA
FAU - Vozilova, Alexandra
AU  - Vozilova A
FAU - Bayankin, Sergey N
AU  - Bayankin SN
FAU - Napier, Bruce A
AU  - Napier BA
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - United States
TA  - Health Phys
JT  - Health physics
JID - 2985093R
RN  - 0 (Radioactive Waste)
RN  - 0 (Water Pollutants, Radioactive)
SB  - IM
MH  - Aged
MH  - Chromosome Aberrations/*radiation effects
MH  - Chromosome Painting/methods
MH  - Electron Spin Resonance Spectroscopy
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Lymphocytes/radiation effects/ultrastructure
MH  - Middle Aged
MH  - Radiation Dosage
MH  - *Radiation Monitoring
MH  - Radioactive Waste
MH  - Tooth/*radiation effects/ultrastructure
MH  - Water Pollutants, Radioactive
EDAT- 2005/01/15 09:00
MHDA- 2005/02/19 09:00
CRDT- 2005/01/15 09:00
PHST- 2005/01/15 09:00 [pubmed]
PHST- 2005/02/19 09:00 [medline]
PHST- 2005/01/15 09:00 [entrez]
AID - 00004032-200502000-00005 [pii]
AID - 10.1097/01.hp.0000146612.69488.9c [doi]
PST - ppublish
SO  - Health Phys. 2005 Feb;88(2):139-53. doi: 10.1097/01.hp.0000146612.69488.9c.