PMID- 15650784
OWN - NLM
STAT- MEDLINE
DCOM- 20060808
LR  - 20061115
IS  - 1003-9279 (Print)
IS  - 1003-9279 (Linking)
VI  - 18
IP  - 4
DP  - 2004 Dec
TI  - [Inhibition of hepatitis C virus gene expression by antisense nucleotide in 
      vitro].
PG  - 341-3
AB  - OBJECTIVE: To study the mechanism of hepatitis C virus (HCV) gene regulation and 
      the inhibitory effect of antisense RNA on HCV gene expression in vitro. METHODS: 
      The hepatoblastoma cell line (HepG2) was co-transfected by recombinant plasmid of 
      antisense RNA complementary to HCV 5' untranslated region (5'UTR)and HCV 5' UTR 
      Directed luciferase (luc) gene expression recombinant plasmid. Meanwhile a 
      reversed HCV 5'UTR recombinant plasmid which can not transcribe as antisense RNA 
      in the cell and a recombinant plasmid in which the luc was regulated by simian 
      virus 40 (sv40) 5'UTR were used as controls respectively. The level of luc gene 
      expression was determined by an enzymatic assay. RESULTS: The antisense RNA which 
      directed to HCV 5'UTRcould obviously knock down the level of luc gene expression 
      and the close-dependent inhibition of antisense RNA was observed at the same 
      time. However the above inhibition was not shown in the cells co-transfected by 
      reversed HCV 5'UTR recombinant plasmid and HCV 5'UTR directed luc gene expression 
      recombinant plasmid. No reduction was observed in luc gene expression level in 
      the cell co-transfected by both antisense RNA recombinant plasmid and SV40 5'UTR 
      directed luc gene expression recombinant plasmid. CONCLUSION: HCV 5'UTR plays an 
      important role in regulation of viral gene expression. The antisense RNA 
      complementary to HCV 5'UTR could effectively inhibit the gene expression 
      regulated by HCV 5'UTR in vitro.
FAU - Li, Yong-nian
AU  - Li YN
AD  - Department of Infectious Diseases, The 323 Hospital of PLA, Xi'an 710054, China. 
      yongnian@pub.xaonline.com
FAU - Yu, Min
AU  - Yu M
FAU - Wu, Wei-qiang
AU  - Wu WQ
FAU - Gao, Jian-xing
AU  - Gao JX
FAU - Wang, Hong
AU  - Wang H
FAU - Ji, Shao-ping
AU  - Ji SP
FAU - Wang, Qin-huan
AU  - Wang QH
FAU - Si, Chong-wen
AU  - Si CW
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi
JT  - Zhonghua shi yan he lin chuang bing du xue za zhi = Zhonghua shiyan he linchuang 
      bingduxue zazhi = Chinese journal of experimental and clinical virology
JID - 9602873
RN  - 0 (5' Untranslated Regions)
RN  - 0 (RNA, Antisense)
RN  - 0 (RNA, Viral)
RN  - 0 (Recombinant Proteins)
RN  - EC 1.13.12.- (Luciferases)
SB  - IM
MH  - 5' Untranslated Regions/*genetics
MH  - Cell Line, Tumor
MH  - Gene Expression Regulation, Viral
MH  - *Genes, Viral
MH  - Hepacivirus/*genetics
MH  - Hepatoblastoma/pathology
MH  - Humans
MH  - Liver Neoplasms/pathology
MH  - Luciferases/*genetics/metabolism
MH  - Plasmids
MH  - RNA, Antisense/*pharmacology
MH  - RNA, Viral/genetics
MH  - Recombinant Proteins/genetics
MH  - Transfection
EDAT- 2005/01/15 09:00
MHDA- 2006/08/09 09:00
CRDT- 2005/01/15 09:00
PHST- 2005/01/15 09:00 [pubmed]
PHST- 2006/08/09 09:00 [medline]
PHST- 2005/01/15 09:00 [entrez]
PST - ppublish
SO  - Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi. 2004 Dec;18(4):341-3.