PMID- 15652180
OWN - NLM
STAT- MEDLINE
DCOM- 20050301
LR  - 20161126
IS  - 0006-3002 (Print)
IS  - 0006-3002 (Linking)
VI  - 1721
IP  - 1-3
DP  - 2005 Jan 18
TI  - Novel nonmatrix-metalloproteinase-mediated collagen degradation.
PG  - 65-72
AB  - Matrix metalloproteinases (MMPs) and their inhibitors have long been believed to 
      play a major role in the collagen loss seen in destructive temporomandibular 
      joint (TMJ) disorders. This project was originally designed to examine the 
      expression of MMPs and the tissue inhibitors of MMPs (TIMPs) by diseased human 
      TMJ synovial fibroblasts and to determine their ability to degrade Type I 
      collagen. Reverse transcriptase-PCR indicated that these TMJ synovial fibroblasts 
      expressed mRNA for multiple MMPs and TIMPs. The collagen degradation assay showed 
      that these TMJ synovial fibroblasts at passage 3 to 8 were capable of digesting 
      the collagen underneath them on collagen-coated plates. This degradation was 
      inhibited by GM6001, a synthetic MMP inhibitor. During passage 8 to 13, these TMJ 
      fibroblasts were able to digest all the collagen in the wells. This degradation 
      was completely inhibited by combining GM6001 and soybean trypsin inhibitor (STI), 
      a serine proteinase inhibitor. The collagen cleavage activity of collected 
      conditioned media was dramatically inhibited by STI but not by 
      1,10-phenanthroline, an MMP inhibitor. The data suggest that these TMJ cells 
      utilize a MMP-dependent pathway and a novel MMP-independent pathway to digest 
      Type I collagen.
FAU - Song, F
AU  - Song F
AD  - Indiana University School of Dentistry, Department of Oral Biology, 1121 West 
      Michigan Street, Room 271, Indianapolis, IN 46202, USA.
FAU - Windsor, L J
AU  - Windsor LJ
LA  - eng
GR  - AR44701/AR/NIAMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
DEP - 20041104
PL  - Netherlands
TA  - Biochim Biophys Acta
JT  - Biochimica et biophysica acta
JID - 0217513
RN  - 0 (Culture Media, Conditioned)
RN  - 9007-34-5 (Collagen)
RN  - EC 3.4.24.- (Matrix Metalloproteinases)
SB  - IM
MH  - Cells, Cultured
MH  - Collagen/*metabolism
MH  - Culture Media, Conditioned
MH  - Fibroblasts/metabolism
MH  - Humans
MH  - Matrix Metalloproteinases/*physiology
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Synovial Membrane/cytology/metabolism
MH  - Temporomandibular Joint/*metabolism
MH  - Temporomandibular Joint Disorders/*metabolism
EDAT- 2005/01/18 09:00
MHDA- 2005/03/02 09:00
CRDT- 2005/01/18 09:00
PHST- 2004/06/28 00:00 [received]
PHST- 2004/10/05 00:00 [revised]
PHST- 2004/10/06 00:00 [accepted]
PHST- 2005/01/18 09:00 [pubmed]
PHST- 2005/03/02 09:00 [medline]
PHST- 2005/01/18 09:00 [entrez]
AID - S0304-4165(04)00247-8 [pii]
AID - 10.1016/j.bbagen.2004.10.007 [doi]
PST - ppublish
SO  - Biochim Biophys Acta. 2005 Jan 18;1721(1-3):65-72. doi: 
      10.1016/j.bbagen.2004.10.007. Epub 2004 Nov 4.