PMID- 15653198
OWN - NLM
STAT- MEDLINE
DCOM- 20050601
LR  - 20191210
IS  - 0301-2115 (Print)
IS  - 0301-2115 (Linking)
VI  - 118
IP  - 2
DP  - 2005 Feb 1
TI  - In utero transplantation of human hematopoetic stem cells into fetal goats under 
      B-type ultrasonographic scan: an experimental model for the study of potential 
      prenatal therapy.
PG  - 170-3
AB  - OBJECTIVE: Using fetal goats as animal models, to establish the methodology of in 
      utero transplantation of human hematopoeitic stem cell (HSC) under B-scan 
      ultrasonographic guidance for prenatal therapy. STUDY DESIGN: Human HSC were 
      directly injected into the peritoneal cavities of the recipient fetal goats at 
      45-55 days of gestation (term: 145 days) under the guidance of B-type ultrasound 
      scan. After birth, the peripheral blood was collected for fluorescence assisted 
      cell sorting (FACS), quantitative real-time PCR and fluorescence in situ 
      hybridization (FISH) to detect and analyze the presence of human cells in the 
      recipients. RESULTS: The 32 recipients were born alive except one miscarriage. To 
      test for the presence of human-goat chimeras, cells from 13 randomly selected 
      transplanted goats were collected. FACS analyses showed the presence of human 
      cells in all the transplanted goats tested. The average proportion of CD34+ cells 
      and GPA+(glycophorin A) cells in the peripheral blood were 1.34 +/- 1.10% and 
      2.80 +/- 2.10%, respectively. No CD34+ or GPA+ cells were found in the 
      non-transplanted goats tested. The results of the quantitative real-time PCR in 
      three engraftment goats were 1.2 x 10(4), 2.9 x 10(4), and 3.2 x 10(4) copies of 
      human GPA DNA per mug of genomic DNA. FISH experiments showed that cells 
      containing human specific alpha-satellite DNA sequence were present in the 
      peripheral blood of the transplanted goats. CONCLUSIONS: The method described 
      herein is safe and reliable, with low miscarriage risk and high chimerism rate. 
      This approach may provide a promising animal model for potential prenatal 
      treatment.
FAU - Zeng, Fanyi
AU  - Zeng F
AD  - Institute of Medical Genetics, Shanghai Children's Hospital, Shanghai Jiao Tong 
      University, 24/1400 West Beijing Road, Shanghai 200040, PR China.
FAU - Chen, Mei-Jue
AU  - Chen MJ
FAU - Huang, Wen-Ying
AU  - Huang WY
FAU - Yan, Jing-Bin
AU  - Yan JB
FAU - Xiao, Yan-Ping
AU  - Xiao YP
FAU - Gong, Zhi-Juan
AU  - Gong ZJ
FAU - Ren, Zhao-Rui
AU  - Ren ZR
FAU - Huang, Shu-Zhen
AU  - Huang SZ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Ireland
TA  - Eur J Obstet Gynecol Reprod Biol
JT  - European journal of obstetrics, gynecology, and reproductive biology
JID - 0375672
RN  - 0 (DNA, Satellite)
RN  - 0 (Glycophorins)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Animals
MH  - Chromosomes, Human, Pair 17/genetics
MH  - DNA/blood
MH  - DNA, Satellite/blood
MH  - Female
MH  - Flow Cytometry
MH  - Gestational Age
MH  - Glycophorins/genetics
MH  - Goats/blood/*embryology
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - *Models, Animal
MH  - Peritoneal Cavity/embryology
MH  - Polymerase Chain Reaction
MH  - Pregnancy
MH  - Stem Cell Transplantation/*methods
MH  - Transplantation Chimera/genetics
MH  - Transplantation, Heterologous
MH  - Ultrasonography/*methods
EDAT- 2005/01/18 09:00
MHDA- 2005/06/02 09:00
CRDT- 2005/01/18 09:00
PHST- 2004/01/14 00:00 [received]
PHST- 2004/04/02 00:00 [revised]
PHST- 2004/05/07 00:00 [accepted]
PHST- 2005/01/18 09:00 [pubmed]
PHST- 2005/06/02 09:00 [medline]
PHST- 2005/01/18 09:00 [entrez]
AID - S0301211504002581 [pii]
AID - 10.1016/j.ejogrb.2004.05.011 [doi]
PST - ppublish
SO  - Eur J Obstet Gynecol Reprod Biol. 2005 Feb 1;118(2):170-3. doi: 
      10.1016/j.ejogrb.2004.05.011.