PMID- 15654229
OWN - NLM
STAT- MEDLINE
DCOM- 20050901
LR  - 20191109
IS  - 1098-3600 (Print)
IS  - 1098-3600 (Linking)
VI  - 7
IP  - 1
DP  - 2005 Jan
TI  - Fluorescence in situ hybridization and chromosome studies after transfusion in 
      newborns: is a waiting period necessary?
PG  - 54-7
AB  - BACKGROUND: Delaying chromosome studies after transfusion is common practice in 
      many neonatal intensive care units (NICUs). Yet, no evidence exists to support 
      this practice. PURPOSE: To investigate the effects of filtration and irradiation 
      on chromosome detection, and to evaluate donor chromosome interference after 
      transfusion. METHODS: Packed red blood cells (PRBCs) were evaluated by 
      fluorescence in situ hybridization (FISH) and chromosome analyses. To evaluate 
      donor leukocyte survival, blood was collected from female neonates who received 
      male-donated PRBCs. RESULTS: Irradiated, leukodepleted blood had no Y chromosome 
      detection by FISH. Irradiated, microaggregate filtered blood had Y chromosome 
      detection in all samples by FISH but no metaphase growth. No donor chromosomes 
      were detected in neonates after transfusion. CONCLUSIONS: Delaying chromosome or 
      FISH analysis in transfused neonates who have received irradiated blood is 
      unnecessary.
FAU - Fynn, Jennifer
AU  - Fynn J
AD  - Advocate Lutheran General Children's Hospital, Department of Pediatrics, Park 
      Ridge, Illinois, USA.
FAU - Komotos, Violeta
AU  - Komotos V
FAU - Rita, Debra
AU  - Rita D
FAU - Benawra, Raghbir
AU  - Benawra R
FAU - Schweig, Lorene
AU  - Schweig L
FAU - Angst, Denise
AU  - Angst D
FAU - Puppala, Bhagya
AU  - Puppala B
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Genet Med
JT  - Genetics in medicine : official journal of the American College of Medical 
      Genetics
JID - 9815831
SB  - IM
MH  - *Chromosomes, Human, X/genetics
MH  - *Chromosomes, Human, Y/genetics
MH  - *Erythrocyte Transfusion
MH  - *Erythrocytes/cytology
MH  - Female
MH  - Graft Survival
MH  - Humans
MH  - *In Situ Hybridization/methods
MH  - Infant, Newborn
MH  - *Karyotyping/methods
MH  - Leukocyte Reduction Procedures/methods
MH  - Leukocytes/cytology
MH  - Male
EDAT- 2005/01/18 09:00
MHDA- 2005/09/02 09:00
CRDT- 2005/01/18 09:00
PHST- 2005/01/18 09:00 [pubmed]
PHST- 2005/09/02 09:00 [medline]
PHST- 2005/01/18 09:00 [entrez]
AID - 00125817-200501000-00009 [pii]
AID - 10.1097/01.gim.0000151151.04087.95 [doi]
PST - ppublish
SO  - Genet Med. 2005 Jan;7(1):54-7. doi: 10.1097/01.gim.0000151151.04087.95.