PMID- 15658605
OWN - NLM
STAT- MEDLINE
DCOM- 20050511
LR  - 20191109
IS  - 0892-3973 (Print)
IS  - 0892-3973 (Linking)
VI  - 26
IP  - 4
DP  - 2004
TI  - Human hemoglobin shares bioactivities ascribed to human tumor necrosis 
      factor-alpha.
PG  - 559-72
AB  - Hemoglobin mediated cytotoxicity and apoptosis has been evaluated in Tumor 
      necrosis factor-alpha (TNF-alpha) sensitive cell line, U937 and compared with 
      TNF-alpha. Both species of hemoglobin, Hemoglobin A2 and Hemoglobin A0 induced 
      apoptosis and cytotoxicity in U937 cell as measured by flow cytometry and 
      3-(4,5-dihydro-6-(4-(3,4-dimethoxybenzooyl)-1-piperazinyl)-2(1H)-quinoline (MTT) 
      assay respectively. Different concentration of Hemoglobin A0 (4 ng/mL to 4000 
      ng/mL) induced apoptosis ranging from 9% to 16% in U937 cells. 4000 ng/mL 
      hemoglobin A0 showed maximal apoptotic cells. TNF-alpha showed 87% apoptotic U937 
      cells at concentration of 1 pg/mL. HbA0 displayed cytotoxicity in U937 cell line 
      at higher concentration in comparison to TNF-alpha. 4000 ng/mL of hemoglobin A0 
      showed optimal cytotoxic response in U937 cells. A dose response curve was also 
      observed with varying doses of hemoglobin A0. U937 cells pretreated with serum 
      activated LPS for 1 hr and incubated with different concentration of hemoglobin 
      or human TNF-alpha for 24 h reduced the cytotoxic effect on U937. Dexamethasone 
      treatment of U937 cells helped in protecting the HbA0 and HbA2 mediated 
      cytotoxicity and anti-TNF-alpha antibody neutralized the hemoglobin mediated 
      apoptosis and cytotoxicity. It is therefore apparent that human hemoglobin shares 
      some of the bioactivities previously ascribed to TNF-alpha. Sharing of 
      bioactivities of TNF-alpha by hemoglobin is interesting and suggests that cell 
      free hemoglobin can mimic TNF-alpha functionally.
FAU - Yadav, Ajay K
AU  - Yadav AK
AD  - Immunobiology Laboratory, Industrial Toxicology Research Centre, Lucknow, India.
FAU - Paul, Bholanath N
AU  - Paul BN
FAU - Naik, Sita
AU  - Naik S
FAU - Saxena, Ashok K
AU  - Saxena AK
FAU - Patel, Devendra Kumar
AU  - Patel DK
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Immunopharmacol Immunotoxicol
JT  - Immunopharmacology and immunotoxicology
JID - 8800150
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 54651-57-9 (hemoglobin A(0))
RN  - 7S5I7G3JQL (Dexamethasone)
RN  - 9034-51-9 (Hemoglobin A)
RN  - 9034-53-1 (Hemoglobin A2)
SB  - IM
MH  - Apoptosis/*drug effects
MH  - Cell Line, Tumor
MH  - Cell Survival/drug effects
MH  - Dexamethasone/pharmacology
MH  - Dose-Response Relationship, Drug
MH  - Hemoglobin A/*pharmacology
MH  - Hemoglobin A2/*pharmacology
MH  - Humans
MH  - Tumor Necrosis Factor-alpha/*pharmacology
EDAT- 2005/01/22 09:00
MHDA- 2005/05/12 09:00
CRDT- 2005/01/22 09:00
PHST- 2005/01/22 09:00 [pubmed]
PHST- 2005/05/12 09:00 [medline]
PHST- 2005/01/22 09:00 [entrez]
AID - 10.1081/iph-200042309 [doi]
PST - ppublish
SO  - Immunopharmacol Immunotoxicol. 2004;26(4):559-72. doi: 10.1081/iph-200042309.