PMID- 15659115
OWN - NLM
STAT- MEDLINE
DCOM- 20050616
LR  - 20161124
IS  - 1671-4083 (Print)
IS  - 1671-4083 (Linking)
VI  - 26
IP  - 1
DP  - 2005 Jan
TI  - Ginsenoside Rg1 reduces MPTP-induced substantia nigra neuron loss by suppressing 
      oxidative stress.
PG  - 56-62
AB  - AIM: To investigate the effect of ginsenoside Rg1, an effective ingredient from 
      ginsenoside, on 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-induced 
      substantia nigra neuron lesion. METHODS: C57-BL mice were given MPTP to prepare 
      Parkinson disease mice model. Different doses of Rg1 (5, 10, and 20 
      mg.kg(-1).d(-1)) or N-acetylcystein (NAC) (300 mg.kg(-1).d(-1)) were given 3 d 
      prior to MPTP in the pretreatment groups. Glutathione (GSH) level and total 
      superoxide dismutase (T-SOD) activity in substantia nigra were determined by 
      spectrophotometry. Nissl staining, tyrosine hydroxylase immunostaining, and TUNEL 
      labeling were used to observe the damage and apoptosis of nigral neurons. Western 
      blot analysis was used to detect the phospho-JNK and phospho-c-Jun levels in 
      midbrain homogenates. RESULTS: Pretreatments of C57-BL mice with different doses 
      of Rg1 or NAC were found to protect against MPTP-induced substantia nigra neurons 
      loss. Rg1 or NAC prevented GSH reduction and T-SOD activation in substantia 
      nigra, and attenuated the phosphorylations of JNK and c-Jun following MPTP 
      treatment. CONCLUSION: The antioxidant property of Rg1 along with the blocking of 
      JNK signaling cascade might contribute to the neuroprotective effect of 
      ginsenoside Rg1 against MPTP.
FAU - Chen, Xiao-chun
AU  - Chen XC
AD  - Fujian Institute of Geriatrics, Union Hospital, Fujian Medical University, Fuzhou 
      350001, China. chenxc998@sohu.com
FAU - Zhou, Yi-can
AU  - Zhou YC
FAU - Chen, Ying
AU  - Chen Y
FAU - Zhu, Yuan-gui
AU  - Zhu YG
FAU - Fang, Fang
AU  - Fang F
FAU - Chen, Li-min
AU  - Chen LM
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Acta Pharmacol Sin
JT  - Acta pharmacologica Sinica
JID - 100956087
RN  - 0 (Ginsenosides)
RN  - 0 (Neuroprotective Agents)
RN  - EC 1.15.1.1 (Superoxide Dismutase)
RN  - EC 2.7.11.24 (JNK Mitogen-Activated Protein Kinases)
RN  - GAN16C9B8O (Glutathione)
RN  - PJ788634QY (ginsenoside Rg1)
SB  - IM
MH  - Animals
MH  - *Apoptosis
MH  - Ginsenosides/isolation & purification/*pharmacology
MH  - Glutathione/metabolism
MH  - JNK Mitogen-Activated Protein Kinases/metabolism
MH  - *MPTP Poisoning/metabolism/pathology
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Neurons/pathology
MH  - Neuroprotective Agents/pharmacology
MH  - Oxidative Stress/*drug effects
MH  - Panax/chemistry
MH  - Plants, Medicinal/chemistry
MH  - *Substantia Nigra/pathology
MH  - Superoxide Dismutase/metabolism
EDAT- 2005/01/22 09:00
MHDA- 2005/06/17 09:00
CRDT- 2005/01/22 09:00
PHST- 2005/01/22 09:00 [pubmed]
PHST- 2005/06/17 09:00 [medline]
PHST- 2005/01/22 09:00 [entrez]
AID - APHS019 [pii]
AID - 10.1111/j.1745-7254.2005.00019.x [doi]
PST - ppublish
SO  - Acta Pharmacol Sin. 2005 Jan;26(1):56-62. doi: 10.1111/j.1745-7254.2005.00019.x.