PMID- 15659185
OWN - NLM
STAT- MEDLINE
DCOM- 20050524
LR  - 20171116
IS  - 1364-5072 (Print)
IS  - 1364-5072 (Linking)
VI  - 98
IP  - 2
DP  - 2005
TI  - Environmental inactivation of Cryptosporidium oocysts in catchment soils.
PG  - 308-17
AB  - AIMS: To generate field-relevant inactivation rates for Cryptosporidium oocysts 
      in soil that may serve as parameter values in models to predict the terrestrial 
      fate and transport of oocysts in catchments. METHODS AND RESULTS: The 
      inactivation of Cryptosporidium oocysts in closed soil microcosms over time was 
      monitored using fluorescence in situ hybridization (FISH) as an estimate of 
      oocyst 'viability'. Inactivation rates for Cryptosporidium in two soils were 
      determined under a range of temperature, moisture and biotic status regimes. 
      Temperature and soil type emerged as significantly influential factors (P < 0.05) 
      for Cryptosporidium inactivation. In particular, temperatures as high as 35 
      degrees C may result in enhanced inactivation. CONCLUSIONS: When modelling the 
      fate of Cryptosporidium oocysts in catchment soils, the use of inactivation rates 
      that are appropriate for the specific catchment climate and soil types is 
      essential. FISH was considered cost-effective and appropriate for determining 
      oocyst inactivation rates in soil. SIGNIFICANCE AND IMPACT OF THE STUDY: Previous 
      models for predicting the fate of pathogens in catchments have either made 
      nonvalidated assumptions regarding inactivation of Cryptosporidium in the 
      terrestrial environment or have not considered it at all. Field-relevant 
      inactivation data are presented, with significant implications for the management 
      of catchments in warm temperate and tropical environments.
FAU - Davies, C M
AU  - Davies CM
AD  - Centre for Water and Waste Technology, School of Civil and Environmental 
      Engineering, University of New South Wales, Sydney, NSW, Australia. 
      c.davies@unsw.edu.au
FAU - Altavilla, N
AU  - Altavilla N
FAU - Krogh, M
AU  - Krogh M
FAU - Ferguson, C M
AU  - Ferguson CM
FAU - Deere, D A
AU  - Deere DA
FAU - Ashbolt, N J
AU  - Ashbolt NJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Appl Microbiol
JT  - Journal of applied microbiology
JID - 9706280
RN  - 0 (Soil)
SB  - IM
MH  - Animals
MH  - *Cryptosporidium
MH  - Environmental Monitoring/methods
MH  - In Situ Hybridization, Fluorescence
MH  - Oocysts
MH  - Soil
MH  - *Soil Microbiology
MH  - Temperature
MH  - Time Factors
MH  - *Water Purification
EDAT- 2005/01/22 09:00
MHDA- 2005/05/25 09:00
CRDT- 2005/01/22 09:00
PHST- 2005/01/22 09:00 [pubmed]
PHST- 2005/05/25 09:00 [medline]
PHST- 2005/01/22 09:00 [entrez]
AID - JAM2459 [pii]
AID - 10.1111/j.1365-2672.2004.02459.x [doi]
PST - ppublish
SO  - J Appl Microbiol. 2005;98(2):308-17. doi: 10.1111/j.1365-2672.2004.02459.x.