PMID- 15659422
OWN - NLM
STAT- MEDLINE
DCOM- 20050328
LR  - 20220129
IS  - 1460-2156 (Electronic)
IS  - 0006-8950 (Print)
IS  - 0006-8950 (Linking)
VI  - 128
IP  - Pt 3
DP  - 2005 Mar
TI  - Early and late HHV-6 gene transcripts in multiple sclerosis lesions and normal 
      appearing white matter.
PG  - 516-27
AB  - Multiple sclerosis is an inflammatory demyelinating disease of the CNS, the 
      aetiology of which is believed to have both genetic and environmental components. 
      We have investigated one of the candidate viruses for the environmental component 
      of multiple sclerosis, the neurotropic human herpesvirus 6 (HHV-6). Utilizing 
      fluorescent in situ hybridization (FISH) techniques, we have examined human 
      post-mortem tissues for the presence of immediate early and late viral gene 
      expression in multiple sclerosis patient normal appearing white matter (NAWM), 
      lesional tissue and normal control brain samples. HHV-6 gene transcription was 
      detected in all tissue samples and was restricted to oligodendrocytes, as 
      determined by double mRNA FISH analysis. Quantitative analysis of viral mRNA 
      expression indicated that both NAWM and lesional multiple sclerosis samples 
      exhibited significantly higher levels of HHV-6 expression compared with the 
      normal control samples. Lesional samples exhibited the highest levels of viral 
      gene expression, with NAWM exhibiting an intermediate level between lesional and 
      control tissues. Immunofluorescence against early and late HHV-6 proteins 
      verified active translation of HHV-6 viral mRNA in oligodendrocytes. Southern 
      blot analysis of nested polymerase chain reactions using extracted genomic DNA 
      and cDNA confirmed the presence of the HHV-6 genome in all individuals, with the 
      active expression profile mirroring the FISH results. The frequent high level of 
      HHV-6 infection in multiple sclerosis samples suggests a possible role in 
      pathogenesis.
FAU - Opsahl, Margaret L
AU  - Opsahl ML
AD  - University of Glasgow Department of Neurology, Division of Clinical 
      Neurosciences, Institute of Neurological Sciences, Southern General Hospital, 
      Glasgow, UK.
FAU - Kennedy, Peter G E
AU  - Kennedy PG
LA  - eng
GR  - 559/MSS_/Multiple Sclerosis Society/United Kingdom
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20050119
PL  - England
TA  - Brain
JT  - Brain : a journal of neurology
JID - 0372537
RN  - 0 (RNA, Messenger)
RN  - 0 (RNA, Viral)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Cryopreservation
MH  - Female
MH  - Gene Expression
MH  - Genes, Viral
MH  - Herpesvirus 6, Human/genetics/*isolation & purification
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Middle Aged
MH  - Multiple Sclerosis/*virology
MH  - Oligodendroglia/virology
MH  - RNA, Messenger/analysis
MH  - RNA, Viral/analysis
MH  - Reverse Transcriptase Polymerase Chain Reaction/methods
MH  - Roseolovirus Infections/*complications/diagnosis
MH  - Transcription, Genetic
PMC - PMC7109784
EDAT- 2005/01/22 09:00
MHDA- 2005/03/29 09:00
PMCR- 2020/04/01
CRDT- 2005/01/22 09:00
PHST- 2005/01/22 09:00 [pubmed]
PHST- 2005/03/29 09:00 [medline]
PHST- 2005/01/22 09:00 [entrez]
PHST- 2020/04/01 00:00 [pmc-release]
AID - awh390 [pii]
AID - 10.1093/brain/awh390 [doi]
PST - ppublish
SO  - Brain. 2005 Mar;128(Pt 3):516-27. doi: 10.1093/brain/awh390. Epub 2005 Jan 19.