PMID- 15659755
OWN - NLM
STAT- MEDLINE
DCOM- 20050309
LR  - 20200306
IS  - 0022-1317 (Print)
IS  - 0022-1317 (Linking)
VI  - 86
IP  - Pt 2
DP  - 2005 Feb
TI  - A subset of human immunodeficiency virus type 1 long-term non-progressors is 
      characterized by the unique presence of ancestral sequences in the viral 
      population.
PG  - 355-364
LID - 10.1099/vir.0.80410-0 [doi]
AB  - Within human immunodeficiency virus type 1 (HIV-1)-infected patients, there are 
      those who have been infected for more than 10 years with a CD4+ cell count of 
      >500 cells microl(-1) and who remain asymptomatic without antiretroviral therapy; 
      these patients are designated long-term non-progressors (LTNPs). In a set of 16 
      LTNPs, viral dating, DNA viral load, quasispecies heterogeneity and antibody (Ab) 
      titres against gp160 and beta2 microglobulin (beta2m) were determined. Plasma 
      viral RNA and CD4+ and CD8+ T-cell numbers were estimated in more than three 
      samples per patient. Host genetic characteristics, such as Delta32-CCR5 genotype 
      and human leukocyte antigen (HLA) genotype and supertypes, and 
      clinical-epidemiological factors were evaluated. Dating of global populations and 
      of DNA and RNA viral quasispecies identified two subsets of patients: one 
      displaying only ancestral sequences and the other displaying predominantly modern 
      sequences. The ancestral patients displayed a significant reduction in RNA and 
      DNA viral loads, quasispecies heterogeneity, CD8+ cell number, anti-gp160 Ab 
      titres and beta2m level, and they were also associated with better use of 
      safe-sex practices and higher presence of the HLA sB58 supertype than the modern 
      subset. Viral dating has therefore permitted the segregation of LTNPs into two 
      subsets that show very different virological, immunological, host and 
      clinical-epidemiological characteristics. Moreover, whereas the modern subset 
      displayed low levels of virus replication, the ancestral group displayed not only 
      a very limited virus replication, often to undetectable levels, but also very 
      slow or arrested viral evolution, maintaining the close relationship of the viral 
      population to the transmitted virus.
FAU - Bello, Gonzalo
AU  - Bello G
AD  - Centro Nacional de Microbiologia, Instituto de Salud Carlos III, Majadahonda, 
      28220 Madrid, Spain.
FAU - Casado, Concepcion
AU  - Casado C
AD  - Centro Nacional de Microbiologia, Instituto de Salud Carlos III, Majadahonda, 
      28220 Madrid, Spain.
FAU - Sandonis, Virginia
AU  - Sandonis V
AD  - Centro Nacional de Microbiologia, Instituto de Salud Carlos III, Majadahonda, 
      28220 Madrid, Spain.
FAU - Alonso-Nieto, Manuela
AU  - Alonso-Nieto M
AD  - Histocompatibilidad, Centro de Transfusion de la Comunidad de Madrid, 28032 
      Madrid, Spain.
FAU - Vicario, Jose Luis
AU  - Vicario JL
AD  - Histocompatibilidad, Centro de Transfusion de la Comunidad de Madrid, 28032 
      Madrid, Spain.
FAU - Garcia, Soledad
AU  - Garcia S
AD  - Centro Sanitario Sandoval, IMSALUD, 28010 Madrid, Spain.
FAU - Hernando, Victoria
AU  - Hernando V
AD  - Centro Sanitario Sandoval, IMSALUD, 28010 Madrid, Spain.
FAU - Rodriguez, Carmen
AU  - Rodriguez C
AD  - Centro Sanitario Sandoval, IMSALUD, 28010 Madrid, Spain.
FAU - Romero, Jorge Del
AU  - Romero JD
AD  - Centro Sanitario Sandoval, IMSALUD, 28010 Madrid, Spain.
FAU - Lopez-Galindez, Cecilio
AU  - Lopez-Galindez C
AD  - Centro Nacional de Microbiologia, Instituto de Salud Carlos III, Majadahonda, 
      28220 Madrid, Spain.
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Gen Virol
JT  - The Journal of general virology
JID - 0077340
RN  - 0 (DNA, Viral)
RN  - 0 (HIV Antibodies)
RN  - 0 (HIV Envelope Protein gp160)
RN  - 0 (HLA Antigens)
RN  - 0 (RNA, Viral)
RN  - 0 (Receptors, CCR5)
RN  - 0 (beta 2-Microglobulin)
SB  - IM
MH  - CD4-CD8 Ratio
MH  - DNA, Viral/analysis
MH  - Genotype
MH  - HIV Antibodies/blood
MH  - HIV Envelope Protein gp160/immunology
MH  - HIV Infections/epidemiology/genetics/*virology
MH  - *HIV Long-Term Survivors
MH  - HIV-1/*genetics
MH  - HLA Antigens/genetics
MH  - Humans
MH  - Phylogeny
MH  - RNA, Viral/analysis
MH  - Receptors, CCR5/genetics
MH  - Safe Sex
MH  - Spain/epidemiology
MH  - Viral Load
MH  - beta 2-Microglobulin/immunology
EDAT- 2005/01/22 09:00
MHDA- 2005/03/10 09:00
CRDT- 2005/01/22 09:00
PHST- 2005/01/22 09:00 [pubmed]
PHST- 2005/03/10 09:00 [medline]
PHST- 2005/01/22 09:00 [entrez]
AID - 10.1099/vir.0.80410-0 [doi]
PST - ppublish
SO  - J Gen Virol. 2005 Feb;86(Pt 2):355-364. doi: 10.1099/vir.0.80410-0.