PMID- 15659840
OWN - NLM
STAT- MEDLINE
DCOM- 20050616
LR  - 20240109
IS  - 0077-8923 (Print)
IS  - 0077-8923 (Linking)
VI  - 1030
DP  - 2004 Dec
TI  - Effects of curcumin (diferuloylmethane) on nuclear factor kappaB signaling in 
      interleukin-1beta-stimulated chondrocytes.
PG  - 578-86
AB  - Curcumin (diferuloylmethane) is a nontoxic dietary pigment in tumeric and curry 
      and a potent inhibitor of the common transcription factor Nuclear Factor kappaB 
      (NF-kappaB) in several cell types. It is well established that some of the 
      catabolic effects of the proinflammatory cytokines interleukin-1beta (IL-1beta) 
      and tumor necrosis factor-alpha in osteoarthritis are regulated by the activation 
      of NF-kappaB. Therefore, the aim of this study was to determine whether curcumin 
      modifies the catabolic response of chondrocytes to IL-1beta. Human articular 
      chondrocytes were prestimulated with 10 ng/mL IL-1beta for 0, 4, 8, 12, or 24 h 
      and then cotreated with 50 microM curcumin for 0, 12, 24, 36, or 48 h. Synthesis 
      of the cartilage-specific collagen type II and matrix-degrading enzyme matrix 
      metalloproteinase-3 (MMP-3) was investigated in chondrocytes by Western blot 
      analysis. Activation and nuclear translocation of NF-kappaB were observed by 
      immunofluorescence microscopy. IL-1beta induced a decrease in collagen type II 
      and upregulation of MMP-3 in a time-dependent manner. Upregulation of MMP-3 was 
      inhibited by curcumin in a time-dependent manner. In addition, IL-1beta-induced a 
      decrease in type II collagen, which was relieved by curcumin treatment. In 
      response to IL-1beta, NF-kappaB translocated to the nucleus, but translocation 
      was inhibited by curcumin, as revealed by immunofluorescence microscopy. Taken 
      together, these results confirmed an IL-1beta-mediated upregulation of 
      proinflammatory MMP-3 in chondrocytes via an NF-kappaB activation mechanism. 
      Curcumin protected chondrocytes from the catabolic effects of IL-1beta, such as 
      MMP-3 upregulation, and interestingly also relieved cytokine-induced suppression 
      of matrix protein synthesis. Therefore, curcumin antagonizes crucial catabolic 
      effects of IL-1beta signaling that are known to contribute to the pathogenesis of 
      osteoarthritis.
FAU - Schulze-Tanzil, Gundula
AU  - Schulze-Tanzil G
AD  - Charite University Medical School Berlin, Institute of Anatomy, Department of 
      Cell and Neurobiology, Campus Benjamin Franklin, Konigin-Luise-Strasse 15, 
      D-14195 Berlin, Germany.
FAU - Mobasheri, Ali
AU  - Mobasheri A
FAU - Sendzik, Judith
AU  - Sendzik J
FAU - John, Thilo
AU  - John T
FAU - Shakibaei, Mehdi
AU  - Shakibaei M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Ann N Y Acad Sci
JT  - Annals of the New York Academy of Sciences
JID - 7506858
RN  - 0 (Interleukin-1)
RN  - 0 (NF-kappa B)
RN  - 9007-34-5 (Collagen)
RN  - EC 3.4.24.17 (Matrix Metalloproteinase 3)
RN  - IT942ZTH98 (Curcumin)
SB  - IM
MH  - Blotting, Western
MH  - Cartilage, Articular/cytology/*drug effects/enzymology/metabolism
MH  - Cell Nucleus/metabolism
MH  - Cells, Cultured
MH  - Chondrocytes/*drug effects/enzymology/metabolism
MH  - Collagen/metabolism
MH  - Curcumin/*pharmacology
MH  - Humans
MH  - Interleukin-1/*pharmacology
MH  - Matrix Metalloproteinase 3/metabolism
MH  - Microscopy, Fluorescence
MH  - NF-kappa B/*metabolism
MH  - Signal Transduction/*drug effects
EDAT- 2005/01/22 09:00
MHDA- 2005/06/17 09:00
CRDT- 2005/01/22 09:00
PHST- 2005/01/22 09:00 [pubmed]
PHST- 2005/06/17 09:00 [medline]
PHST- 2005/01/22 09:00 [entrez]
AID - 1030/1/578 [pii]
AID - 10.1196/annals.1329.067 [doi]
PST - ppublish
SO  - Ann N Y Acad Sci. 2004 Dec;1030:578-86. doi: 10.1196/annals.1329.067.