PMID- 15662137
OWN - NLM
STAT- MEDLINE
DCOM- 20050726
LR  - 20200930
IS  - 1538-4047 (Print)
IS  - 1538-4047 (Linking)
VI  - 3
IP  - 12
DP  - 2004 Dec
TI  - RNA interference: a potential strategy for isoform-specific phosphatidylinositol 
      3-kinase targeted therapy in ovarian cancer.
PG  - 1283-9
AB  - Phosphatidylinositol 3-kinase (PI3-kinase) is a novel intracellular transducer 
      involved in a wide range of cancer-associated signaling pathways, which comprises 
      various isoforms and splice variants with distinct biologic activities and 
      clinical implications. Especially, the class Ia PI3-kinase 110 kD catalytic 
      subunit alpha (PIK3CA) is the most important isoform in tumorigenesis and 
      possibly, tumor angiogenesis. Several strategies have been developed to block 
      PI3-kinase for cancer therapy; however, the approach to target specific 
      PI3-kinase isoform has not been explored to date. In the present study, we show 
      that RNA interference (RNAi) through small interfering (siRNA) sequences 
      targeting PIK3CA has potential applications in isoform-specific "knock-down" of 
      PI3-kinase. This strategy provides a novel tool to study the function of various 
      PI3-kinase isoforms and may contribute to isoform-specific targeting of 
      PI3-kinase in human cancer.
FAU - Zhang, Lin
AU  - Zhang L
AD  - Abramson Family Cancer Research Institute, University of Pennsylvania School of 
      Medicine, Philadelphia, Pennsylvania 19104, USA.
FAU - Yang, Nuo
AU  - Yang N
FAU - Liang, Shun
AU  - Liang S
FAU - Barchetti, Andrea
AU  - Barchetti A
FAU - Vezzani, Cristina
AU  - Vezzani C
FAU - Huang, Jia
AU  - Huang J
FAU - O'Brien-Jenkins, Ann
AU  - O'Brien-Jenkins A
FAU - Rubin, Stephen C
AU  - Rubin SC
FAU - Coukos, George
AU  - Coukos G
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20041215
PL  - United States
TA  - Cancer Biol Ther
JT  - Cancer biology & therapy
JID - 101137842
RN  - 0 (Biomarkers, Tumor)
RN  - 0 (Phosphoinositide-3 Kinase Inhibitors)
RN  - 0 (Protein Isoforms)
RN  - 0 (RNA, Small Interfering)
RN  - EC 2.7.1.137 (Class I Phosphatidylinositol 3-Kinases)
RN  - EC 2.7.1.137 (PIK3CA protein, human)
SB  - IM
MH  - Animals
MH  - Biomarkers, Tumor/metabolism
MH  - Blotting, Western
MH  - *Cell Proliferation
MH  - Class I Phosphatidylinositol 3-Kinases
MH  - Female
MH  - *Genetic Vectors
MH  - Humans
MH  - Mice
MH  - Ovarian Neoplasms/*genetics/metabolism
MH  - Phosphatidylinositol 3-Kinases/*genetics/metabolism
MH  - Phosphoinositide-3 Kinase Inhibitors
MH  - Protein Isoforms
MH  - RNA Interference
MH  - RNA, Small Interfering/*pharmacology
MH  - Tumor Cells, Cultured
EDAT- 2005/01/22 09:00
MHDA- 2005/07/27 09:00
CRDT- 2005/01/22 09:00
PHST- 2005/01/22 09:00 [pubmed]
PHST- 2005/07/27 09:00 [medline]
PHST- 2005/01/22 09:00 [entrez]
AID - 1388 [pii]
AID - 10.4161/cbt.3.12.1388 [doi]
PST - ppublish
SO  - Cancer Biol Ther. 2004 Dec;3(12):1283-9. doi: 10.4161/cbt.3.12.1388. Epub 2004 
      Dec 15.