PMID- 15663742
OWN - NLM
STAT- MEDLINE
DCOM- 20050602
LR  - 20111117
IS  - 0001-2815 (Print)
IS  - 0001-2815 (Linking)
VI  - 65
IP  - 1
DP  - 2005 Jan
TI  - Allelic polymorphism in introns 1 and 2 of the HLA-DQA1 gene.
PG  - 56-66
AB  - Human leukocyte antigen (HLA) class II antigens are highly polymorphic membrane 
      glycoproteins, encoded by the A and B genes of DR, DQ, and DP. The polymorphism 
      is mainly located in exon 2, with the exception of DQA1. Of the 27 DQA1 alleles 
      presently known, 18 cannot be identified on the basis of exon 2 alone, but need 
      additional information from the other exons. DQA1 has been reported to be the 
      most ancient class II gene. For evolutionary comparison and to assess the degree 
      of polymorphism outside the exons, the sequences of introns 1 and 2 were 
      determined from 30 different cell lines, encompassing 15 different DQA1 alleles. 
      The sequences revealed major nucleotide differences between the different 
      lineages, whereas within each lineage few differences were present. Phylogenetic 
      analysis of intron and exon sequences confirmed this lineage specificity. 
      Altogether, the present data indicate that the HLA-DQA1 lineages represent 
      ancient entities. The observed variation of the introns in alleles with identical 
      exon sequences implicates conservative selection of the exons within a given 
      lineage. Intron sequences may provide the means to set up an accurate typing 
      system.
FAU - Voorter, C E M
AU  - Voorter CE
AD  - Tissue Typing Laboratory, University Hospital Maastricht, Maastricht, The 
      Netherlands.
FAU - de Groot, N G
AU  - de Groot NG
FAU - Meertens, C M H
AU  - Meertens CM
FAU - Bontrop, R E
AU  - Bontrop RE
FAU - van den Berg-Loonen, E M
AU  - van den Berg-Loonen EM
LA  - eng
PT  - Journal Article
PL  - England
TA  - Tissue Antigens
JT  - Tissue antigens
JID - 0331072
RN  - 0 (HLA-DQ Antigens)
RN  - 0 (HLA-DQ alpha-Chains)
RN  - 0 (HLA-DQA1 antigen)
SB  - IM
MH  - Base Sequence
MH  - Cell Line
MH  - Exons
MH  - HLA-DQ Antigens/*genetics/immunology
MH  - HLA-DQ alpha-Chains
MH  - Humans
MH  - Introns
MH  - Molecular Sequence Data
MH  - *Polymorphism, Genetic
EDAT- 2005/01/25 09:00
MHDA- 2005/06/03 09:00
CRDT- 2005/01/25 09:00
PHST- 2005/01/25 09:00 [pubmed]
PHST- 2005/06/03 09:00 [medline]
PHST- 2005/01/25 09:00 [entrez]
AID - TAN344 [pii]
AID - 10.1111/j.1399-0039.2005.00344.x [doi]
PST - ppublish
SO  - Tissue Antigens. 2005 Jan;65(1):56-66. doi: 10.1111/j.1399-0039.2005.00344.x.