PMID- 15663747
OWN - NLM
STAT- MEDLINE
DCOM- 20050602
LR  - 20081121
IS  - 0001-2815 (Print)
IS  - 0001-2815 (Linking)
VI  - 65
IP  - 1
DP  - 2005 Jan
TI  - Emerging new alleles suggest high diversity of HLA-C locus.
PG  - 101-6
AB  - Human leukocyte antigen (HLA)-C has only recently emerged as an important 
      transplantation antigen and as a receptor for natural killer cells. Over the last 
      few years, sequence-based typing (SBT) revealed the true diversity of HLA-C 
      locus; however, the frequency at which new alleles are detected still remains 
      high. During routine SBT of 3500 samples for the National Marrow Donor Program, 
      we have identified 20 new HLA-C alleles reported in this article in 26 
      individuals. New variants have been characterized by direct sequencing of 
      polymerase chain reaction product obtained by allele-specific amplification of 
      potential new alleles. Most of the new alleles carry coding substitutions of 
      residues located within the antigen-binding groove. The substitutions are 
      predominantly located in the alpha2-helix which is consistent with the unique to 
      HLA-C conservation of alpha1-helix. Seven new alleles, or 35%, have been 
      identified in African Americans, two of them in three and four individuals each, 
      suggesting that these alleles may not be rare. This observation reflects the fact 
      that the minority groups, previously under-represented in the HLA research pools 
      subjected to SBT, now begin to emerge as a main source of new HLA-C alleles. This 
      study further confirms that HLA-C locus is at least as polymorphic as HLA-A and 
      HLA-B.
FAU - Lebedeva, T V
AU  - Lebedeva TV
AD  - HLA Laboratory, American Red Cross Blood Services, New England Region, Dedham, MA 
      02026, USA. lebedeva@redcross.org
FAU - Ohashi, M
AU  - Ohashi M
FAU - Huang, A
AU  - Huang A
FAU - Vasconcellos, S
AU  - Vasconcellos S
FAU - Flesch, S
AU  - Flesch S
FAU - Yu, N
AU  - Yu N
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - England
TA  - Tissue Antigens
JT  - Tissue antigens
JID - 0331072
RN  - 0 (HLA-C Antigens)
RN  - 0 (Receptors, Antigen, T-Cell)
SB  - IM
MH  - Alleles
MH  - Binding Sites
MH  - Genetic Variation
MH  - HLA-C Antigens/*genetics/immunology
MH  - Humans
MH  - Receptors, Antigen, T-Cell/immunology
EDAT- 2005/01/25 09:00
MHDA- 2005/06/03 09:00
CRDT- 2005/01/25 09:00
PHST- 2005/01/25 09:00 [pubmed]
PHST- 2005/06/03 09:00 [medline]
PHST- 2005/01/25 09:00 [entrez]
AID - TAN333 [pii]
AID - 10.1111/j.1399-0039.2005.00333.x [doi]
PST - ppublish
SO  - Tissue Antigens. 2005 Jan;65(1):101-6. doi: 10.1111/j.1399-0039.2005.00333.x.