PMID- 15664045
OWN - NLM
STAT- MEDLINE
DCOM- 20050317
LR  - 20050124
IS  - 0166-0934 (Print)
IS  - 0166-0934 (Linking)
VI  - 124
IP  - 1-2
DP  - 2005 Mar
TI  - Real-time PCR for quantitation of porcine reproductive and respiratory syndrome 
      virus and porcine circovirus type 2 in naturally-infected and challenged pigs.
PG  - 11-9
AB  - Real-time PCR assays were developed for quantitative detection of porcine 
      reproductive and respiratory syndrome virus (PRRSV) and porcine circovirus type 2 
      (PCV2). The established real-time PCR for the quantitation of PRRSV cDNA and PCV2 
      DNA were found to be in the 9-log(10) linear dynamic range with excellent 
      linearity and reliable reproducibility. Using these techniques, the distribution 
      and quantitation of PRRSV and PCV2 in naturally infected and challenged pigs were 
      investigated. The viral concentrations were expressed as the mean log(10) viral 
      DNA or cDNA copy numbers per mg or ml of tested samples. For pigs infected 
      naturally with both viruses, the lung, spleen, tonsil and lymphoid organs had the 
      highest viral burdens with ranges from 5.73 to 8.38 and 5.65 to 6.91 for PRRSV 
      and PCV2, respectively. The injection of formalin-inactivated Salmonella 
      choleraesuis emulsified in complete Freund's adjuvant 1 week before and after the 
      inoculation of both viruses resulted in PRRSV replication enhancement 2 weeks 
      post-challenge. However, this facilitated the clearance of PRRSV 4 weeks 
      post-challenge. Results from this study show that the established quantitative 
      PCR could be a useful tool when applied to vaccine development and pathogenesis 
      studies in the future.
FAU - Chung, Wen-Bin
AU  - Chung WB
AD  - Department of Veterinary Medicine, National Pingtung University of Science and 
      Technology, Pingtung 912, ROC, Taiwan. wbchung@mail.npust.edu.tw
FAU - Chan, Wen-Hung
AU  - Chan WH
FAU - Chaung, Hso-Chi
AU  - Chaung HC
FAU - Lien, Yi
AU  - Lien Y
FAU - Wu, Chia-Chang
AU  - Wu CC
FAU - Huang, Yu-Liang
AU  - Huang YL
LA  - eng
PT  - Journal Article
DEP - 20041128
PL  - Netherlands
TA  - J Virol Methods
JT  - Journal of virological methods
JID - 8005839
RN  - 0 (DNA, Viral)
SB  - IM
MH  - Animals
MH  - Circoviridae Infections/*veterinary/virology
MH  - Circovirus/genetics/*isolation & purification
MH  - DNA, Viral/analysis
MH  - Polymerase Chain Reaction/*methods
MH  - Porcine Reproductive and Respiratory Syndrome/*virology
MH  - Porcine respiratory and reproductive syndrome virus/genetics/*isolation & 
      purification
MH  - Reproducibility of Results
MH  - Swine/virology
MH  - Swine Diseases/*virology
EDAT- 2005/01/25 09:00
MHDA- 2005/03/18 09:00
CRDT- 2005/01/25 09:00
PHST- 2004/07/01 00:00 [received]
PHST- 2004/10/12 00:00 [revised]
PHST- 2004/10/18 00:00 [accepted]
PHST- 2005/01/25 09:00 [pubmed]
PHST- 2005/03/18 09:00 [medline]
PHST- 2005/01/25 09:00 [entrez]
AID - S0166-0934(04)00308-8 [pii]
AID - 10.1016/j.jviromet.2004.10.003 [doi]
PST - ppublish
SO  - J Virol Methods. 2005 Mar;124(1-2):11-9. doi: 10.1016/j.jviromet.2004.10.003. 
      Epub 2004 Nov 28.