PMID- 15664729
OWN - NLM
STAT- MEDLINE
DCOM- 20050525
LR  - 20131121
IS  - 0531-5565 (Print)
IS  - 0531-5565 (Linking)
VI  - 40
IP  - 1-2
DP  - 2005 Jan-Feb
TI  - Effects of long-term caloric restriction on glucose homeostasis and on the first 
      steps of the insulin signaling system in skeletal muscle of normal and Ames dwarf 
      (Prop1df/Prop1df) mice.
PG  - 27-35
AB  - Ames dwarf mice are a model of retarded aging and extended longevity and display 
      enhanced insulin sensitivity. Caloric restriction (CR) and the dwarf mutation 
      have additive effects on lifespan. To begin to understand the mechanisms behind 
      this effect, an analysis of the in vivo status of the insulin signaling system 
      was performed in skeletal muscle from Ames dwarf (df/df) and normal mice fed ad 
      libitum or subjected to long-term (over 1 year) CR. The response to CR was 
      different in both groups of animals. In normal animals, CR induced a significant 
      reduction in both circulating insulin and glucose levels, together with an 
      increase in the in vivo insulin-stimulated phosphorylation of the IR, a trend 
      towards an increase in the in vivo insulin-stimulated phosphorylation levels of 
      IR substrate-1, and an increase in the abundance of GLUT4 in muscle. In contrast, 
      CR did not modify none of these parameters in df/df mice. Interestingly, CR 
      induced a reduction in the p85 subunit of phosphatidylinositol 3-kinase abundance 
      in skeletal muscle in both groups of animals. These results suggest that in 
      skeletal muscle, long-term CR induces different effects on the first steps of the 
      insulin signaling system in normal mice than in df/df mice.
FAU - Argentino, Danila Paula
AU  - Argentino DP
AD  - Facultad de Farmacia y Bioquimica, Instituto de Quimica y Fisicoquimica 
      Biologicas (UBA-CONICET), Junin 956, 1113AAD Buenos Aires, Argentina.
FAU - Dominici, Fernando Pablo
AU  - Dominici FP
FAU - Munoz, Marina Cecilia
AU  - Munoz MC
FAU - Al-Regaiey, Khalid
AU  - Al-Regaiey K
FAU - Bartke, Andrzej
AU  - Bartke A
FAU - Turyn, Daniel
AU  - Turyn D
LA  - eng
GR  - AG 19899/AG/NIA NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Exp Gerontol
JT  - Experimental gerontology
JID - 0047061
RN  - 0 (Blood Glucose)
RN  - 0 (Glucose Transporter Type 4)
RN  - 0 (Insulin)
RN  - 0 (Insulin Receptor Substrate Proteins)
RN  - 0 (Irs1 protein, mouse)
RN  - 0 (Monosaccharide Transport Proteins)
RN  - 0 (Muscle Proteins)
RN  - 0 (Phosphoproteins)
RN  - 0 (Slc2a4 protein, mouse)
RN  - 42HK56048U (Tyrosine)
RN  - EC 2.7.1.- (Phosphatidylinositol 3-Kinases)
SB  - IM
MH  - Aging/metabolism
MH  - Animals
MH  - Blood Glucose/*metabolism
MH  - Body Weight/physiology
MH  - *Caloric Restriction
MH  - Dwarfism/metabolism
MH  - Female
MH  - Glucose Transporter Type 4
MH  - Insulin/blood/*physiology
MH  - Insulin Receptor Substrate Proteins
MH  - Longevity/physiology
MH  - Mice
MH  - Mice, Mutant Strains
MH  - Monosaccharide Transport Proteins/metabolism
MH  - Muscle Proteins/metabolism
MH  - Muscle, Skeletal/*metabolism
MH  - Phosphatidylinositol 3-Kinases/metabolism
MH  - Phosphoproteins/metabolism
MH  - Phosphorylation
MH  - Signal Transduction/physiology
MH  - Tyrosine/metabolism
EDAT- 2005/01/25 09:00
MHDA- 2005/05/26 09:00
CRDT- 2005/01/25 09:00
PHST- 2004/07/15 00:00 [received]
PHST- 2004/08/31 00:00 [revised]
PHST- 2004/09/13 00:00 [accepted]
PHST- 2005/01/25 09:00 [pubmed]
PHST- 2005/05/26 09:00 [medline]
PHST- 2005/01/25 09:00 [entrez]
AID - S0531-5565(04)00297-9 [pii]
AID - 10.1016/j.exger.2004.09.005 [doi]
PST - ppublish
SO  - Exp Gerontol. 2005 Jan-Feb;40(1-2):27-35. doi: 10.1016/j.exger.2004.09.005.