PMID- 15664957
OWN - NLM
STAT- MEDLINE
DCOM- 20050311
LR  - 20181113
IS  - 0019-9567 (Print)
IS  - 1098-5522 (Electronic)
IS  - 0019-9567 (Linking)
VI  - 73
IP  - 2
DP  - 2005 Feb
TI  - Antibody-mediated protection against Cryptococcus neoformans pulmonary infection 
      is dependent on B cells.
PG  - 1141-50
AB  - The pathogenesis of pulmonary Cryptococcus neoformans infection and the efficacy 
      of passive immunoglobulin G1 (IgG1) administration were investigated in 
      B-cell-deficient and C57BL/6J mice. C57BL/6J mice lived longer than 
      B-cell-deficient mice after both intratracheal and intravenous infections. 
      Administration of IgG1 prior to infection prolonged the survival of C57BL/6J mice 
      but had no effect on the survival or numbers of CFU in the lungs of 
      B-cell-deficient mice. C. neoformans infection in B-cell-deficient mice resulted 
      in significantly higher levels of gamma interferon (IFN-gamma), monocyte 
      chemoattractant protein-1 (MCP-1), and macrophage inflammatory protein-1alpha 
      (MIP-1alpha) than in C57BL/6J mice. IgG1 administration reduced IFN-gamma and 
      MCP-1 levels in C57BL/6J mice but not in B-cell-deficient mice. In addition, 
      compared to its effect in C57BL/6J mice, C. neoformans infection in 
      FcRgammaIII-deficient, athymic, and SCID mice significantly increased IFN-gamma 
      and MCP-1 levels. IgG1 administration was associated with reduced IFN-gamma 
      levels in C57BL/6J mice but not in FcRgammaIII-deficient, athymic, and SCID mice. 
      These observations suggest that IgG1-mediated protection in this system is a 
      consequence of alterations in the inflammatory response that translate into less 
      damage to the host without directly reducing the fungal burden. For hosts with 
      impaired immunities, the ineffectiveness of passive antibody (Ab) may reflect an 
      inability to down-regulate inflammation and avoid self-damage. The results 
      indicate an important role for B cells in host defense against C. neoformans 
      infection and demonstrate a surprising dependence of Ab-mediated protection on B 
      cells in this system.
FAU - Rivera, Johanna
AU  - Rivera J
AD  - Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, NY 10461, 
      USA.
FAU - Zaragoza, Oscar
AU  - Zaragoza O
FAU - Casadevall, Arturo
AU  - Casadevall A
LA  - eng
GR  - HL 59842/HL/NHLBI NIH HHS/United States
GR  - R01 AI033142/AI/NIAID NIH HHS/United States
GR  - T32 CA009173/CA/NCI NIH HHS/United States
GR  - 5T32 CA 09173/CA/NCI NIH HHS/United States
GR  - R01 AI033774/AI/NIAID NIH HHS/United States
GR  - R01 HL059842/HL/NHLBI NIH HHS/United States
GR  - AI 33774/AI/NIAID NIH HHS/United States
GR  - R37 AI033142/AI/NIAID NIH HHS/United States
GR  - AI 33142/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Infect Immun
JT  - Infection and immunity
JID - 0246127
RN  - 0 (Antibodies)
RN  - 0 (Cytokines)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Animals
MH  - Antibodies/administration & dosage/*immunology
MH  - B-Lymphocytes/*immunology
MH  - Cryptococcosis/*immunology
MH  - Cryptococcus neoformans/*immunology
MH  - Cytokines/*metabolism
MH  - Flow Cytometry
MH  - Immunization, Passive
MH  - Immunoglobulin E/blood
MH  - Lung/cytology/microbiology
MH  - Mice
MH  - Survival
PMC - PMC546959
EDAT- 2005/01/25 09:00
MHDA- 2005/03/12 09:00
PMCR- 2005/02/01
CRDT- 2005/01/25 09:00
PHST- 2005/01/25 09:00 [pubmed]
PHST- 2005/03/12 09:00 [medline]
PHST- 2005/01/25 09:00 [entrez]
PHST- 2005/02/01 00:00 [pmc-release]
AID - 73/2/1141 [pii]
AID - 0809-04 [pii]
AID - 10.1128/IAI.73.2.1141-1150.2005 [doi]
PST - ppublish
SO  - Infect Immun. 2005 Feb;73(2):1141-50. doi: 10.1128/IAI.73.2.1141-1150.2005.