PMID- 15665470 OWN - NLM STAT- MEDLINE DCOM- 20050511 LR - 20211203 IS - 0916-8451 (Print) IS - 0916-8451 (Linking) VI - 69 IP - 1 DP - 2005 Jan TI - Isolation and characterization of a cDNA encoding phytochrome A in the non-photosynthetic parasitic plant, Orobanche minor Sm. PG - 71-8 AB - In this study, the isolation and characterization of a phytochrome A (PHYA) homologous cDNA (OmPHYA) in the non-photosynthetic holoparasitic plant Orobanche minor are described. The present findings provide the first report of the presence of a PHYA homolog in the holoparasite. This study found that OmPHYA is of similar size to the other PHYAs of green plants and shows 72, 77, and 77% amino acid sequence identity with PHYA in Arabidopsis, potato, and tobacco respectively. The OmPHYA contains a conserved chromophore attachment cysteine at position 323. Although OmPHYA shows high sequence identity with other PHYAs in green plants, 13 amino acid substitutions located in both the N and C-terminal domains are observed (a total of 26 amino acids). OmPHYA is encoded by a single gene within the O. minor genome. The abundance of the OmPHYA transcript as well as nuclear translocation of OmphyA occurs in a light-dependent manner. FAU - Trakulnaleamsai, Chitra AU - Trakulnaleamsai C AD - Department of Biotechnology, Graduate School of Engineering, Osaka University, Osaka, Japan. FAU - Okazawa, Atsushi AU - Okazawa A FAU - An, Chung-Il AU - An CI FAU - Kajiyama, Shin'ichiro AU - Kajiyama S FAU - Fukusaki, Ei'ichiro AU - Fukusaki E FAU - Yoneyama, Koichi AU - Yoneyama K FAU - Takeuchi, Yasutomo AU - Takeuchi Y FAU - Kobayashi, Akio AU - Kobayashi A LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't PL - England TA - Biosci Biotechnol Biochem JT - Bioscience, biotechnology, and biochemistry JID - 9205717 RN - 0 (Arabidopsis Proteins) RN - 0 (DNA, Complementary) RN - 0 (DNA, Plant) RN - 0 (PHYA protein, Arabidopsis) RN - 0 (Phytochrome A) RN - 11121-56-5 (Phytochrome) RN - EC 2.7.11.1 (Protein Serine-Threonine Kinases) SB - IM MH - Amino Acid Sequence MH - Arabidopsis Proteins MH - DNA, Complementary/chemistry/*isolation & purification MH - DNA, Plant/chemistry/*isolation & purification MH - Gene Expression Regulation, Plant MH - Molecular Sequence Data MH - Orobanche/genetics/*physiology MH - Phylogeny MH - Phytochrome/*chemistry/genetics/*physiology MH - Phytochrome A MH - Protein Serine-Threonine Kinases/*chemistry/genetics/*physiology MH - Sequence Homology, Amino Acid EDAT- 2005/01/25 09:00 MHDA- 2005/05/12 09:00 CRDT- 2005/01/25 09:00 PHST- 2005/01/25 09:00 [pubmed] PHST- 2005/05/12 09:00 [medline] PHST- 2005/01/25 09:00 [entrez] AID - JST.JSTAGE/bbb/69.71 [pii] AID - 10.1271/bbb.69.71 [doi] PST - ppublish SO - Biosci Biotechnol Biochem. 2005 Jan;69(1):71-8. doi: 10.1271/bbb.69.71.