PMID- 15668209
OWN - NLM
STAT- MEDLINE
DCOM- 20050419
LR  - 20181201
IS  - 0032-0781 (Print)
IS  - 0032-0781 (Linking)
VI  - 46
IP  - 1
DP  - 2005 Jan
TI  - Symbiotic rhizobium and nitric oxide induce gene expression of non-symbiotic 
      hemoglobin in Lotus japonicus.
PG  - 99-107
AB  - We characterized the expression profiles of LjHb1 and LjHb2, non-symbiotic 
      hemoglobin (non-sym-Hb) genes of Lotus japonicus. Although LjHb1 and LjHb2 showed 
      77% homology in their cDNA sequences, LjHb2 is located in a unique position in 
      the phylogenetic tree of plant Hbs. The 5'-upstream regions of both genes contain 
      the motif AAAGGG at a position similar to that in promoters of other non-sym-Hb 
      genes. Expression profiles obtained by using quantitative RT-PCR showed that 
      LjHb1 and LjHb2 were expressed in all tissues of mature plants, and expression 
      was enhanced in mature root nodules. LjHb1 was strongly induced under both 
      hypoxic and cold conditions, and by the application of nitric oxide (NO) donor, 
      whereas LjHb2 was induced only by the application of sucrose. LjHb1 was also 
      induced transiently by the inoculation with the symbiotic rhizobium Mesorhizobium 
      loti MAFF303099. Observations using fluorescence microscopy revealed the 
      induction of LjHb1 expression corresponded to the generation of NO. These results 
      suggest that non-sym-Hb and NO have important roles in stress adaptation and in 
      the early stage of legume-rhizobium symbiosis.
FAU - Shimoda, Yoshikazu
AU  - Shimoda Y
AD  - Graduate School of Science and Technology, Kagoshima University, Kagoshima, 
      890-0065 Japan.
FAU - Nagata, Maki
AU  - Nagata M
FAU - Suzuki, Akihiro
AU  - Suzuki A
FAU - Abe, Mikiko
AU  - Abe M
FAU - Sato, Shusei
AU  - Sato S
FAU - Kato, Tomohiko
AU  - Kato T
FAU - Tabata, Satoshi
AU  - Tabata S
FAU - Higashi, Shiro
AU  - Higashi S
FAU - Uchiumi, Toshiki
AU  - Uchiumi T
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20050124
PL  - Japan
TA  - Plant Cell Physiol
JT  - Plant & cell physiology
JID - 9430925
RN  - 0 (DNA, Plant)
RN  - 0 (Hemoglobins)
RN  - 0 (Nitric Oxide Donors)
RN  - 0 (Plant Growth Regulators)
RN  - 0 (Plant Proteins)
RN  - 31C4KY9ESH (Nitric Oxide)
SB  - IM
MH  - Alphaproteobacteria/*metabolism
MH  - Base Sequence
MH  - DNA, Plant/genetics
MH  - Gene Expression
MH  - Gene Expression Regulation, Plant/drug effects
MH  - Genes, Plant
MH  - Hemoglobins/*genetics
MH  - Lotus/drug effects/*genetics/metabolism/microbiology
MH  - Nitric Oxide/*metabolism
MH  - Nitric Oxide Donors/pharmacology
MH  - Phylogeny
MH  - Plant Growth Regulators/pharmacology
MH  - Plant Proteins/genetics
MH  - Promoter Regions, Genetic
MH  - Symbiosis
EDAT- 2005/01/26 09:00
MHDA- 2005/04/20 09:00
CRDT- 2005/01/26 09:00
PHST- 2005/01/26 09:00 [pubmed]
PHST- 2005/04/20 09:00 [medline]
PHST- 2005/01/26 09:00 [entrez]
AID - pci001 [pii]
AID - 10.1093/pci/pci001 [doi]
PST - ppublish
SO  - Plant Cell Physiol. 2005 Jan;46(1):99-107. doi: 10.1093/pci/pci001. Epub 2005 Jan 
      24.