PMID- 15668987
OWN - NLM
STAT- MEDLINE
DCOM- 20050823
LR  - 20171116
IS  - 1552-4949 (Print)
IS  - 1552-4949 (Linking)
VI  - 64
IP  - 1
DP  - 2005 Mar
TI  - Flow cytometric analysis of in vitro activated basophils, specific IgE and skin 
      tests in the diagnosis of pollen-associated food allergy.
PG  - 28-33
AB  - BACKGROUND: Specific immunoglobulin E (IgE) and commercially available skin prick 
      tests have been demonstrated to be unreliable methods to diagnose 
      pollen-associated food allergy. To evaluate the predictive value of the basophil 
      activation test (BAT) in pollen-associated food allergy, the apple-mediated oral 
      allergy syndrome (OAS) in patients with birch pollinosis was chosen as a 
      representative model. METHODS: Patients with birch pollen allergy and a history 
      of apple-mediated OAS (OAS(+), n = 29), patients with birch allergic without OAS 
      (OAS(-), n = 22), and healthy controls (HC, n = 10) without birch pollen allergy 
      and OAS were included. Apple IgE was quantified by the CAP FEIA method. Skin 
      prick tests were performed with a Jonagold apple extract. Flow cytometric 
      analysis of basophils activated with the same Jonagold extract was based on 
      double staining with anti-IgE/anti-CD63 monoclonal antibodies. RESULTS: 
      Comparison between OAS(+) subjects and HC showed sensitivities and specificities 
      of 96% and 100% for apple IgE and 88% and 100% for the apple skin prick test, 
      respectively. For the BAT, sensitivity and specificity were 100%. In contrast, 
      when nonresponders on the BAT were considered, sensitivity decreased to 90%. In a 
      separate analysis between OAS(+) and OAS(-) subjects, specificities decreased to 
      30% for apple IgE and to 80% for the apple skin test, respectively. The BAT 
      reached a sensitivity of 88% and a specificity of 75%. CONCLUSION: Flow 
      cytometry-assisted quantification of in vitro basophil activation seems to be a 
      reliable instrument in the diagnosis of this model of pollen-associated food 
      allergy. In addition, this study reemphasizes that the specificity of diagnostic 
      allergy tests decreases considerably when, apart from HC, control individuals 
      with cross-reactive antibodies are included.
FAU - Ebo, Didier G
AU  - Ebo DG
AD  - Department of Immunology, Allergology, and Rheumatology, University of Antwerp, 
      Antwerp, Belgium.
FAU - Hagendorens, Margo M
AU  - Hagendorens MM
FAU - Bridts, Chris H
AU  - Bridts CH
FAU - Schuerwegh, Annemie J
AU  - Schuerwegh AJ
FAU - De Clerck, Luc S
AU  - De Clerck LS
FAU - Stevens, Wim J
AU  - Stevens WJ
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Cytometry B Clin Cytom
JT  - Cytometry. Part B, Clinical cytometry
JID - 101235690
RN  - 0 (Antigens, CD)
RN  - 0 (CD63 protein, human)
RN  - 0 (Platelet Membrane Glycoproteins)
RN  - 0 (Tetraspanin 30)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Antigens, CD/metabolism
MH  - Basophils/*immunology/metabolism
MH  - Betula/immunology
MH  - Female
MH  - Flow Cytometry/*methods
MH  - Food Hypersensitivity/*diagnosis/immunology
MH  - Humans
MH  - Immunoglobulin E/*blood
MH  - Male
MH  - Malus/immunology
MH  - Middle Aged
MH  - Platelet Membrane Glycoproteins/metabolism
MH  - Pollen/*immunology
MH  - ROC Curve
MH  - Sensitivity and Specificity
MH  - Skin Tests
MH  - Tetraspanin 30
MH  - Up-Regulation/immunology
EDAT- 2005/01/26 09:00
MHDA- 2005/08/24 09:00
CRDT- 2005/01/26 09:00
PHST- 2005/01/26 09:00 [pubmed]
PHST- 2005/08/24 09:00 [medline]
PHST- 2005/01/26 09:00 [entrez]
AID - 10.1002/cyto.b.20042 [doi]
PST - ppublish
SO  - Cytometry B Clin Cytom. 2005 Mar;64(1):28-33. doi: 10.1002/cyto.b.20042.