PMID- 15670271
OWN - NLM
STAT- MEDLINE
DCOM- 20050309
LR  - 20061115
IS  - 1350-1925 (Print)
IS  - 1350-1925 (Linking)
VI  - 17
IP  - 1
DP  - 2005 Feb
TI  - Hypersensitivity to ovalbumin induces chronic intestinal dysmotility and 
      increases the number of intestinal mast cells.
PG  - 112-22
AB  - Undiagnosed food allergies have been proposed as possible causes of promoting and 
      perpetuating irritable bowel syndrome . Our aim was to find out if sensitization 
      could induce chronic functional motor disturbances in the intestine and the 
      mechanisms implicated. Rats were sensitized to ovalbumin (OVA) following three 
      hypersensitivity induction protocols, two parenteral and one oral. Rat mast cell 
      protease II (RMCP II) release in response to OVA challenge and immunoglobulin E 
      (IgE) concentration were measured in serum. At least 1 week after challenge, 
      small intestinal motility was evaluated using strain gauges. Intestinal tissue 
      samples from orally sensitized rats were checked for in vitro stimulation with 
      OVA. Mucosal mast cells were counted from duodenum sections. All sensitized rats 
      showed intestinal hypermotility. Only rats sensitized by parenteral procedure 
      showed an increase in RMCP II after OVA challenge in serum. IgEs increased only 
      in the Bordetella pertussis sensitized group. Small intestine sections from 
      orally sensitized rats released more RMCP II than sections from control rats. All 
      sensitized rats showed an increase in the number of mucosal mast cells in 
      duodenum. In conclusion, hypersensitivity to food proteins induces chronic motor 
      alteration that persists long after antigen challenge and an excited/activated 
      state of sensitized mucosal mast cells.
FAU - Saavedra, Y
AU  - Saavedra Y
AD  - Department of Cell Biology, Physiology and Immunology, Universitat Autonoma de 
      Barcelona, Bellaterra, Spain.
FAU - Vergara, P
AU  - Vergara P
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Neurogastroenterol Motil
JT  - Neurogastroenterology and motility
JID - 9432572
RN  - 37341-29-0 (Immunoglobulin E)
RN  - 9006-59-1 (Ovalbumin)
RN  - 9011-97-6 (Cholecystokinin)
RN  - EC 3.4.21.- (Serine Endopeptidases)
RN  - EC 3.4.21.- (cytotoxic T lymphocyte-specific serine protease)
SB  - IM
MH  - Animals
MH  - Bordetella pertussis/immunology
MH  - Cholecystokinin/pharmacology
MH  - Duodenum/pathology/physiopathology
MH  - Food Hypersensitivity/*complications/physiopathology
MH  - Gastrointestinal Motility/drug effects/*physiology
MH  - Ileum/pathology/physiopathology
MH  - Immunoglobulin E/analysis/biosynthesis
MH  - Immunohistochemistry
MH  - Intestinal Diseases/*etiology/pathology/physiopathology
MH  - Intestinal Mucosa/pathology
MH  - Intestine, Small/pathology/physiopathology
MH  - Male
MH  - Mast Cells/*physiology
MH  - Muscle Tonus/drug effects
MH  - Ovalbumin/*immunology
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Serine Endopeptidases/metabolism
EDAT- 2005/01/27 09:00
MHDA- 2005/03/10 09:00
CRDT- 2005/01/27 09:00
PHST- 2005/01/27 09:00 [pubmed]
PHST- 2005/03/10 09:00 [medline]
PHST- 2005/01/27 09:00 [entrez]
AID - NMO597 [pii]
AID - 10.1111/j.1365-2982.2004.00597.x [doi]
PST - ppublish
SO  - Neurogastroenterol Motil. 2005 Feb;17(1):112-22. doi: 
      10.1111/j.1365-2982.2004.00597.x.