PMID- 15670306
OWN - NLM
STAT- MEDLINE
DCOM- 20050623
LR  - 20181030
IS  - 1048-891X (Print)
IS  - 1048-891X (Linking)
VI  - 15
IP  - 1
DP  - 2005 Jan-Feb
TI  - Gain of chromosome 3q is an early and consistent genetic aberration in carcinomas 
      of the vulva.
PG  - 120-6
AB  - The aim was to determine whether specific gains of chromosome 3q and 
      laminin-5gamma2-chain expression can improve early detection of invasive capacity 
      in precancerous and squamous cell carcinoma of the vulva (VSCC). Six VSCC and 
      three precancerous lesions were studied. Multicolor fluorescence in situ 
      hybridization (FISH) probe sets were applied to nuclei suspensions prepared from 
      archival material using the Hedley method. The probe panel consists of the 
      centromers of chromosome 7, chromosome 3, and the TERC gene residing on the long 
      arm of chromosome 3. Laminin-5gamma2-chain immunohistochemical analysis was 
      performed on corresponding specimens and was expressed only in the VSCC. The 
      genome-specific FISH analysis revealed 3q amplification in 43% of the nuclei 
      analyzed for the VSCC and 22% of the nuclei for the precancerous lesions. 
      Low-level 3q amplifications were found in precancerous lesions with an average 
      fold increase of 1.15 for 3q. The invasive lesions showed higher average fold 
      increases for 3q, averaging 1.32. Laminin-5gamma2-chain protein was expressed 
      only in VSCC, whereas 3q gains were observed both in precancerous lesions and in 
      VSCC, indicating that gain of chromosome 3q is an early and consistent event 
      during carcinogenesis of VSCC.
FAU - Stoltzfus, P
AU  - Stoltzfus P
AD  - Department of Oncology and Pathology, Karolinska Hospital, 171-76 Stockholm, 
      Sweden.
FAU - Heselmeyer-Haddad, K
AU  - Heselmeyer-Haddad K
FAU - Castro, J
AU  - Castro J
FAU - White, N
AU  - White N
FAU - Silfversward, C
AU  - Silfversward C
FAU - Sjovall, K
AU  - Sjovall K
FAU - Einhorn, N
AU  - Einhorn N
FAU - Tryggvason, K
AU  - Tryggvason K
FAU - Auer, G
AU  - Auer G
FAU - Ried, T
AU  - Ried T
FAU - Nordstrom, B
AU  - Nordstrom B
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Int J Gynecol Cancer
JT  - International journal of gynecological cancer : official journal of the 
      International Gynecological Cancer Society
JID - 9111626
RN  - 0 (Biomarkers, Tumor)
RN  - 0 (LAMC2 protein, human)
RN  - 0 (Laminin)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Biomarkers, Tumor
MH  - Carcinoma, Squamous Cell/*genetics/pathology
MH  - *Chromosome Aberrations
MH  - Chromosomes, Human, Pair 3/*genetics
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Laminin/biosynthesis/genetics
MH  - Middle Aged
MH  - Neoplasm Invasiveness/genetics
MH  - Precancerous Conditions/*genetics/pathology
MH  - Vulvar Neoplasms/*genetics/pathology
EDAT- 2005/01/27 09:00
MHDA- 2005/06/24 09:00
CRDT- 2005/01/27 09:00
PHST- 2005/01/27 09:00 [pubmed]
PHST- 2005/06/24 09:00 [medline]
PHST- 2005/01/27 09:00 [entrez]
AID - IJG15012 [pii]
AID - 10.1111/j.1048-891X.2005.15012.x [doi]
PST - ppublish
SO  - Int J Gynecol Cancer. 2005 Jan-Feb;15(1):120-6. doi: 
      10.1111/j.1048-891X.2005.15012.x.