PMID- 15671274
OWN - NLM
STAT- MEDLINE
DCOM- 20050311
LR  - 20171116
IS  - 0146-0404 (Print)
IS  - 0146-0404 (Linking)
VI  - 46
IP  - 2
DP  - 2005 Feb
TI  - Characterization and distribution of bone marrow-derived cells in mouse cornea.
PG  - 497-503
AB  - PURPOSE: Bone marrow (BM)-derived stem cells are thought to possess extensive 
      differentiation capacity. The present study was conducted to investigate the 
      characteristics and distribution of these cells in the normal mouse cornea. 
      METHODS: BM cells and BM-derived hematopoietic stem/progenitor cells (HSCs) from 
      enhanced GFP (eGFP) transgenic mice (lin(-), Sca-1(+)) were intravenously 
      transplanted into irradiated wild-type C57BL/6 mice. At 4 to 6 months after 
      transplantation, the mice were killed, and their whole corneas examined by 
      histologic and immunohistochemical methods (CD11c, CD11b, and CD45). RESULTS: At 
      2 weeks after BM cell transplantation, GFP(+) cells gradually migrated into the 
      cornea from the limbal area. At 2 to 6 months, they were distributed over the 
      entire cornea. In cross sections of whole cornea, GFP(+) cells comprised 27.3% 
      +/- 11.1% (BM) and 24.0% +/- 8.01% (HSC) of total cells in the peripheral corneal 
      stroma. In the center of the corneal stroma, GFP(+) cells were 7.58% +/- 2.63% 
      (BM) and 8.06% +/- 1.76% (HSC) of total cells. Immunohistochemistry showed that 
      GFP(+) CD11c(+), CD11b(+), CD11c(-), and CD11b(-) cells occupied the entire 
      corneal stroma. CONCLUSIONS: The present study provides direct evidence of the 
      distribution of BM-derived cells in the mouse cornea. Immunohistochemical study 
      showed that some of these cells are BM-derived antigen-presenting cells such as 
      dendritic cells and macrophages. Some elements of BM-derived cells may continue 
      to exist in the corneal stroma.
FAU - Nakamura, Takahiro
AU  - Nakamura T
AD  - Department of Ophthalmology, Kyoto Prefectural University of Medicine, Kyoto 
      602-0841, Japan. tnakamur@ophth.kpu-m.ac.jp
FAU - Ishikawa, Fumihiko
AU  - Ishikawa F
FAU - Sonoda, Koh-hei
AU  - Sonoda KH
FAU - Hisatomi, Toshio
AU  - Hisatomi T
FAU - Qiao, Hong
AU  - Qiao H
FAU - Yamada, Jun
AU  - Yamada J
FAU - Fukata, Mitsuhiro
AU  - Fukata M
FAU - Ishibashi, Tatsuro
AU  - Ishibashi T
FAU - Harada, Mine
AU  - Harada M
FAU - Kinoshita, Shigeru
AU  - Kinoshita S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Invest Ophthalmol Vis Sci
JT  - Investigative ophthalmology & visual science
JID - 7703701
RN  - 0 (CD11 Antigens)
RN  - 0 (enhanced green fluorescent protein)
RN  - 147336-22-9 (Green Fluorescent Proteins)
SB  - IM
MH  - Animals
MH  - Animals, Newborn
MH  - Bone Marrow Cells/*cytology/metabolism
MH  - Bone Marrow Transplantation
MH  - CD11 Antigens/metabolism
MH  - Cell Movement/physiology
MH  - Cornea/*cytology/metabolism
MH  - Flow Cytometry
MH  - Green Fluorescent Proteins/metabolism
MH  - Hematopoietic Stem Cell Transplantation
MH  - Hematopoietic Stem Cells/*cytology/metabolism
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Transgenic
EDAT- 2005/01/27 09:00
MHDA- 2005/03/12 09:00
CRDT- 2005/01/27 09:00
PHST- 2005/01/27 09:00 [pubmed]
PHST- 2005/03/12 09:00 [medline]
PHST- 2005/01/27 09:00 [entrez]
AID - 46/2/497 [pii]
AID - 10.1167/iovs.04-1154 [doi]
PST - ppublish
SO  - Invest Ophthalmol Vis Sci. 2005 Feb;46(2):497-503. doi: 10.1167/iovs.04-1154.